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Bioaccumulation: aquatic / sediment:

From CompTox Chemistry Dashboard using OPERA (OPEn (quantitative) structure-activity Relationship Application)  V1.02 model in which calculation based on PaDEL descriptors (calculate molecular descriptors and fingerprints of chemical)  the bioaccumulation i.e BCF for test substance 3,7-bis(diethylamino)phenoxazin-5-ium acetate was estimated to be 54.6 dimensionless. The predicted BCF result based on the 5 OECD principles. Thus based on the result it is concluded that the test substance 3,7-bis(diethylamino)phenoxazin-5-ium acetate is non-bioaccumulative in nature

Additional information

Bioaccumulation: aquatic / sediment:

Various predicted data for the target compound 3,7-bis(diethylamino)phenoxazin -5-ium acetate (CAS No. 79916 -07 -7) and supporting weight of evidence study for its read across substance were reviewed for the bioaccumulation end point which are summarized as below:

 

In a prediction done using CompTox Chemistry Dashboard using OPERA (OPEn (quantitative) structure-activity Relationship Application)  V1.02 model in which calculation based on PaDEL descriptors (calculate molecular descriptors and fingerprints of chemical)  the bioaccumulation i.e BCF for test substance 3,7-bis(diethylamino)phenoxazin-5-ium acetate was estimated to be 54.6 dimensionless. The predicted BCF result based on the 5 OECD principles. Thus based on the result it is concluded that the test substance 3,7-bis(diethylamino)phenoxazin-5-ium acetate is non-bioaccumulative in nature.

 

In a supporting weight of evidence study from authoritative database (J-check, 2017) for the read across chemical 3',6'Bis(diethylamino)spiro[isobenzofuran1(3H),9'[9H]xanthene]3one (CAS no 509-34-2) Bioaccumulation test was conducted for 28 days for determination the bioconcentration factor (BCF) and lipid content of test substance 3',6'Bis(diethylamino)spiro[isobenzofuran1(3H),9'[9H]xanthene]3one (Cas no.509-34-2) on test organism Cyprinus carpio. The purity of test substance by HPLC and LCMS was found to be 99.6% and water solubility value is 851mg/l at 25ᵒC .The recovery ratio is given-Test water: 97.8 % and Fish: 91.9 %  and Nominal concentrations used in the study as - 1st concentration area: 10 microg/L, 2nd concentration area: 1 microg/L and Range finding study was carried out on Rice fish (Oryzias latipes) and the 96 hr LC50 value was found to be > 10.0 mg/L. By analytical methods, the limit of quantification was for Test water: (Fish: 9.3 ng/g). 1st concentration area: 0.18 µg/l, 2nd concentration area: 0.018µg/l . Whereas the lipid content at the start and end of exposure was found to be 2.87% and 1.94%, respectively. Thus according to guideline OECD Guideline 305 (Bioconcentration: Flow through Fish Test) the BCF value for test substance 3',6'Bis(diethylamino)spiro [isobenzofuran1(3H),9'[9H]xanthene]3one (Cas no.509-34-2) was observed to be <= 0.91 L/kg at dose concentration 10 microg/L and <= 9.1 L/kg at dose concentration 1 microg/L on test organism Cyprinus carpio during 28 day period. These values indicate that the substance is not bioaccumulative.

 

Bioaccumulation test was conducted for 6 weeks for determination of the bioconcentration factor (BCF) and lipid content of test substance Rhodamine B (81-88-9) from j-check, 2017 on test organism Cyprinus carpio. The purity of test substance was 99% and water solubility value is 10 g/L at 25ᵒC. The Recovery ratio of test water in 1st concentration area was 93.0 %, in 2nd concentration area was 94.4 %, and in Fish 85.2 %. In the Limit of detection Test water concentration in 1st concentration area was 3.0 ng/mL, in 2nd concentration area was 0.30 ng/mL, and in Fish 16 ng/g was taken. Nominal concentrations used in the study as1st Concentration area was 0.1 mg/L, and2nd Concentration area was 0.01 mg/L. whereas the lipid content at the start was found to be 3.9 %. The bioconcentration factor (BCF value) of substance 2-((2-hydroxy-1-naphthalenyl)azo)-5-((4-sulfophenyl)azo)-benzenesulfonic acid, disodium salt (Rhodamine B) on Cyprinus carpio was determined to be ≤ 0.2 L/kg at a conc. of 0.1 mg/l and ≤ 1.7 L/Kg at a conc. of 0.01 mg/l, respectively. These values indicate that the substance is not bioaccumulative.

 

On the basis of available information, the test 3,7-bis(diethylamino)phenoxazin-5-ium acetate (79916-07-7) can be considered to be non bioaccumulative in nature.