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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2017
Report date:
2017

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes

Test material

Constituent 1
Reference substance name:
Reaction products of diazotized 4-amino-6-[(4-aminophenyl)diazenyl]-3-[(4-aminophenyl)diazenyl]-5-hydroxynaphthalene-2,7-disulfonic acid, coupled with benzene-1,3-diamine, sodium salts
EC Number:
812-037-7
Cas Number:
1793011-72-9
Molecular formula:
Unknown for all components
IUPAC Name:
Reaction products of diazotized 4-amino-6-[(4-aminophenyl)diazenyl]-3-[(4-aminophenyl)diazenyl]-5-hydroxynaphthalene-2,7-disulfonic acid, coupled with benzene-1,3-diamine, sodium salts
Test material form:
solid

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
- Concentrations: 100 mg/L (nominal)
- Sampling method: One set was collected (fresh and old medium). Samples were taken from the middle layer of the relevant vessels.

Test solutions

Vehicle:
no
Details on test solutions:
As the test substance was soluble a 100 mg/L solution was prepared by adding the appropriate amount of substance to water in a 1 L volumetric flask. This was then inverted and stirred for 5 minutes.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna
- Source: In house cultures
- Feeding during test: No

ACCLIMATION
- Acclimation period: 28 days or less
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: Chlorella vulgaris 6 mgC (organic carbon) /2 L/day
- Feeding frequency: As above
- Health during acclimation (any mortality observed): None observed

METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES: Juveniles less than 24 hours old were used for the test.

Study design

Test type:
semi-static
Water media type:
saltwater
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Hardness:
Not specified
Test temperature:
20°C in test and control replicates
pH:
Within the range 7.7 and 8.2.
Dissolved oxygen:
Within the range 8.5 and 8.9 D.O mg/L
Salinity:
Not specified
Conductivity:
Not specified
Nominal and measured concentrations:
Nominal loading rate: 100 mg/L
Mean measured: 99.9 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 100 mL vessel
- Type: open
- Aeration: None
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Elendt M4 media as per OECD guideline

OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 hour light/ 8 hour dark
- Light intensity: 800 Lux or less

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Immobility (lack of movement within 15 seconds of gentle agitation) assessed at 24 and 48 hours.

RANGE-FINDING STUDY
- Test concentrations: 0 and 100 mg/L
- Results used to determine the conditions for the definitive study: Yes , no evidence of immobilisation observed in the range-finder.
Reference substance (positive control):
yes
Remarks:
Potassium dichromate test conducted every 6 months

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
No immobility was observed at a limit concentration of 100 mg/L. The mean test concentration at this concentration was 99.9 mg/L. The test solutions used on study were black with no precipitate evident although roughly 50% or more of the vessel surfaces were covered in an oily slick.
Results with reference substance (positive control):
- Results with reference substance valid: Yes (within historical laboratory range)
Reported statistics and error estimates:
Not applicable as a limit test

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Remarks:
validity criteria on test fulfilled
Conclusions:
Under the conditions of the test no immobility was observed at the limit concentration of 100 mg/L to Daphnia magna and accordingly the 48 hour EC50 value was > 100 mg/L. The measured concentrations were near nominal.