Trimethyl[2-(phosphonooxy)ethyl]ammonium chloride, calcium salt (1:1)

Ecotoxicological Summary

Hazard for aquatic organisms

Freshwater

Hazard assessment conclusion:

PNEC aqua (freshwater)

PNEC value:

0.1 mg/L

Assessment factor:

1 000

Extrapolation method:

assessment factor

PNEC freshwater (intermittent releases):

1 mg/L

Marine water

Hazard assessment conclusion:

PNEC aqua (marine water)

PNEC value:

0.01 mg/L

Assessment factor:

10 000

Extrapolation method:

assessment factor

PNEC marine water (intermittent releases):

0.1 mg/L

STP

Hazard assessment conclusion:

PNEC STP

PNEC value:

10 mg/L

Assessment factor:

10

Extrapolation method:

assessment factor

Sediment (freshwater)

Hazard assessment conclusion:

PNEC sediment (freshwater)

PNEC value:

0.46 mg/kg sediment dw

Extrapolation method:

equilibrium partitioning method

Sediment (marine water)

Hazard assessment conclusion:

PNEC sediment (marine water)

PNEC value:

0.05 mg/kg sediment dw

Extrapolation method:

equilibrium partitioning method

Hazard for air

Air

Hazard assessment conclusion:

no hazard identified

Hazard for terrestrial organisms

Soil

Hazard assessment conclusion:

PNEC soil

PNEC value:

0.03 mg/kg soil dw

Extrapolation method:

equilibrium partitioning method

Hazard for predators

Secondary poisoning

Hazard assessment conclusion:

no potential for bioaccumulation

Additional information

The available data refer to the degradation products of calcium phosphorylcholine chloride (choline and inorganic phosphate):

Short-term toxicity to fish:

- Key study. Method according to OECD 203. GLP study. The 96h-LC50 of the substance choline chloride in Oryzias latipes was higher than 100 mg/L (nominal), based on mortality.

- Key study. Method according to OECD 203. GLP study. The 96h-LC50 of the substance calcium hydrogenorthophosphate in Oryzias latipes was above 100 mg/L (nominal) and the NOEC was 100 mg/L, both based on mortality.

Short-term toxicity to aquatic invertebrates:

- Key study. Method according to OECD 202. GLP study.The 48h-EC50 of choline chloride in Daphnia magna was determined to be 349 mg/L (nominal) and the 48h-NOEC was determined to be 180 mg/L (nominal), both based on mobility.

- Key study. Method according OECD to 202. GLP study.The 48h-EC50 of calcium hydrogenorthophosphate in Daphnia magna was higher than 100 mg/L (nominal) and 2.9 mg/L (measured), based on mobility.

Long-term toxicity to aquatic invertebrates

- Key study. OECD 211. GLP study.The 21 day-EC50 of choline chloride in Daphnia magna was 58.9 mg/L and the 21 day-NOEC was 30.2 mg/L.

Toxicity to aquatic algae and cyanobacteria

- Key study. OECD 201. GLP study.The 72h-EC50 (based on growth rate reduction) of choline chloride in Pseudokichneriella subcapitata was greater than 1000 mg/L and the 72h-NOEC was 1000 mg/L.

- Key study. OECD 201. GLP study.The 72h-EC50 of calcium hydrogenorthophosphate in Pseudokirchneriella subcapitata was found to be higher than 100 mg/L (nominal) and the NOEC was determined to be 100 mg/L, both based on growth rate.

Toxicity to microorganisms

- Key study. Method according OECD 301. The test item was not inhibitory to microorganisms (93.5% biodegradation in 14d) at a concentration of 100 mg/L. Therefore, the NOEC value for the test item in microorganisms is greater than 100 mg/L.

Based on this information, it can be concluded that the substance phosphoryl choline chloride has an 96h-LC50in Oryzias latipes above 100 mg/L, a 48h-EC50 in Daphnia magna higher than 100 mg/L, a 72h-EC50 in Pseudokirchneriella subcapitata higher than 100 mg/L and a NOEC for microorganism toxicity of 100 mg/L.

Conclusion on classification

Based on available data (96h-EC50 in fish > 100 mg/L, 48h-EC50 in Daphnids > 100 mg/L, 72h-EC50 in algae > 100 mg/L) and since the substance is readily biodegradable, with a Log Kow < 4, the test item is not classified for aquatic toxicity, according to CLP regulation (EC) No. 1272/2008.