2-pyridylamine

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

2008

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Remarks:

No further details.

Details on sampling:

The concentration of the test substance in the test solution was measured at the beginning and the end of the exposure, and the average value was 97 ~ 100 % of the set concentration.

Vehicle:

not specified

Test organisms (species):

Oryzias latipes

Details on test organisms:

TEST ORGANISM
- Common name: himedaka
- Strain: Oryzias latipes

Test type:

not specified

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Reference substance (positive control):

not specified

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

11 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Validity criteria fulfilled:

not specified

Conclusions:

The 96h-EC50 of the test item in fish is 11 mg/L.

Executive summary:

An acute fish toxicity test was conducted according to OECD 203 under GLP conditions. The test item concentration was measured at the beginning and at the end of the exposure time, and remained within 97 -100% of the nominal value. The 96h-EC50 for the test item to medaka (Oryzias latipes) was determined to be 11 mg/L, based on the average value of the measured concentration.

Reference 2

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Qualifier:

according to guideline

Guideline:

other: JIS K0102

Version / remarks:

Japanese Industrial Standard K 0102 [1971] (as shown in Annex of OECD 305C)

Deviations:

no

Principles of method if other than guideline:

Median tolerance limit test (TLm) test was carried out according to the procedure of Japan Industrial Standards (Japanese Standards Committee, 1971).

GLP compliance:

not specified

Analytical monitoring:

yes

Vehicle:

yes

Details on test solutions:

- Chemical substances were dissolved in water and neutralized with 0.01N NaOH or HCl, if necessary. Substances which were difficult to dissolve in water were dissolved in 1ml of ethanol, and then diluted in water.

Test organisms (species):

Oryzias latipes

Details on test organisms:

TEST ORGANISM
- Common name: Himedaka (Oryzias Latipes)
- Age at study initiation: same age.
- Length: 2 cm.
- Weight: 0.2 g.

ACCLIMATION
- Acclimation period: 10 days.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Remarks on exposure duration:

After 24 or 48 hours, lethal concentration of 50% fish was determined.

Test temperature:

25ºC

pH:

7

Nominal and measured concentrations:

Not specified.

Details on test conditions:

TEST SYSTEM
- Fill volume: 2 L
- No. of organisms per vessel: 10 fish per trial.

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Deionized water.

OTHER TEST CONDITIONS
Chemical substance wa dissolved in water an neutralized with 0.01N NaOH or HCl if necessary.

EFFECT PARAMETERS MEASURED: Lethal concentration of 50% fish was determined.

Reference substance (positive control):

not required

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

6 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

12 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Other biological observations: not specified.

Results with reference substance (positive control):

The death rate of fish in 0.5 ppm of mercuric chloride (as Hg) should not be more than 20%, while that in 1 ppm should be more than 20% after 24h.

Sublethal observations / clinical signs:

Table1. TLm values and partition coefficients of the aromatic nitrogen compound: 2 -aminopyridine

Substance	TLm (mg/L)^(a)		Partition coeficient(b)
	24 h	48 h
2-Aminopyridine	12.0	6.0	3.00

a) Ten fish of Himedaka were used in 2 L of the solution.

b) Partition coefficient between n-octanol and water.

Validity criteria fulfilled:

not applicable

Conclusions:

The 24 and 48h-LC50 was 12.0 and 6.0 mg/L respectively with the test subtance 2-aminopyridine.

Executive summary:

A median tolerance limit (TLm) test was conducted with the test item. The test was conducted according to the procedure of Japan Industrial Standards JIS K0102 (Japanese Standards Committee, 1971). TLm is equivalent to LC50 and it was calculated for 24 and 48 hours of exposure. The 24h-LC50 was 12.0 mg/L and the 48h-LC50 was 6.0 mg/L. Moreover, the partition coefficient between n-octanol and water was calculated and it was concluded that the larger solubility of compounds in n-octanol was, the larger was the toxicity to fish.

Description of key information

Weight of evidence: A study according to OECD 203 (GLP study) reports a 96h-EC50 = 11 mg/L. A study according to Japan industrial Standards (JIS-K0102, 1971), reports a 48h-EC50 = 6.0 mg/L. Based on the available data, the 96h-EC50 for freswhater fish is 11 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

11 mg/L

Additional information

Weight of evidence. One study performed according to OECD 203 (GLP study), without detailed documentation, reports the 96h-EC50 of the test item in freshwater fish Himedaka (Oryzias latipes) as 11 mg/L, based on the average value of the measured concentrations (measured at the start and at the end of expoure). Another study performed according to Japan industrial Standards (JIS-K0102, 1971), reports the 48h-EC50 of the test item in Himedaka (Oryzias latipes) as 6.0 mg/L. According to OECD 203, 96 hour exposure is the preferred data. Based on the available data, the 96h-EC50 for freswhater fish is 11 mg/L.