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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1987-10-30 to 1988-01-26
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988
Report date:
1988

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: DIN 28 412, part 15
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
2-[[4-(diethylamino)-2-methylphenyl]azo]-5-nitrobenzene-1,3-dicarbonitrile
EC Number:
284-395-7
EC Name:
2-[[4-(diethylamino)-2-methylphenyl]azo]-5-nitrobenzene-1,3-dicarbonitrile
Cas Number:
84870-65-5
Molecular formula:
C19H18N6O2
IUPAC Name:
2-[[4-(diethylamino)-2-methylphenyl]azo]-5-nitrobenzene-1,3-dicarbonitrile
Test material form:
solid

Sampling and analysis

Analytical monitoring:
not specified

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Leuciscus idus melanotus
Details on test organisms:
TEST ORGANISM
- Common name: Goldorfe
- Length at study initiation (length definition, mean, range and SD): 5 - 8cm

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Hardness:
15 +/- 3°
Test temperature:
20 +/- 1°C
pH:
at test start: 8.5
Dissolved oxygen:
> 5mg O2/L
Nominal and measured concentrations:
5 g/L
Details on test conditions:
TEST SYSTEM
- Test vessel: filled volume: 10L
- No. of organisms per vessel: 10
- Biomass loading rate: 0.8 - 1.1 g/cm³

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: mixture of chlor free mains water and deionised water
- Ca/Mg ratio:4:1

OTHER TEST CONDITIONS
- Photoperiod: 12 - 16h light per day

Results and discussion

Effect concentrations
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 5 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
not determinable
Details on results:
Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: product is nearly insoluble, sedimented

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
The test item caused no mortality during the 48 h test. The test item is nearly insoluble in the test medium.