4'-cyanoacetophenone

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data is from publication

Data source

Reference

Reference Type:

publication

Title:

The Sensitization Potential of Some Perfume Ingredients Tested Using a Modified Draize Procedure

Author:

D.W. Sharp

Year:

1978

Bibliographic source:

Toxicology, 9 (1978) 261-271

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Refer below principle

Principles of method if other than guideline:

Preliminary irritation study of Acetophenone (CAS No: 98-86-2) were performed in guinea pigs to determine the concentration for suitable the sensitization study.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Acetophenone

EC Number:

202-708-7

EC Name:

Acetophenone

Cas Number:

98-86-2

Molecular formula:

C8H8O

IUPAC Name:

1-phenylethan-1-one

Test material form:

liquid

Details on test material:

Name of the test chemical:1-phenylethanone (Acetophenone)
Molecular Formula:C8H8O
Molecular Weigth:120.15 g/mol
Substance Type: Organic
Physical State: Liquid

Specific details on test material used for the study:

- Name of test material (as cited in study report): Acetophenone
- Molecular formula: C8H8O
- Molecular weight: 120.148 g/mol
- Substance type: Organic
- Physical state: Liquid
-Smiles: c1(ccccc1)C(=O)C
- InChI: 1S/C8H8O/c1-7(9)8-5-3-2-4-6-8/h2-6H,1H3

Test animals

Species:

guinea pig

Strain:

not specified

Details on test animals or test system and environmental conditions:

- Weight at study initiation:450 g

Test system

Type of coverage:

open

Preparation of test site:

other: Intradermal application: clipped and shaved flanks; Dermal application: shaved flanks

Vehicle:

other: Vehicle used but details were not available.

Controls:

not specified

Amount / concentration applied:

Intradermal application: 0.1ml
Dermal application: Amount- 0.1ml
concentration applied -0.25% ICC and 20%ACC

Duration of treatment / exposure:

24 hours

Observation period:

24 hours

Number of animals:

4 guinea pigs(same sex)

Details on study design:

- Area of exposure: small circular areas

Results and discussion

In vivo

Results

Irritation parameter:

other: Intradermal application: erythema and edema Dermal application: erythema

Basis:

mean

Time point:

24 h

Reversibility:

not specified

Remarks on result:

no indication of irritation

Irritant / corrosive response data:

No skin irritating effects were observed.

Applicant's summary and conclusion

Interpretation of results:

other: Not irritating

Conclusions:

No signs of erythema and edema were observed at 0.25% ICC and 20% ACC concentrations. Hence the test material Acetophenone (CAS No: 98-86-2) was considered to be non-irritating on guinea pigs in the preliminary irritation tests performed for the sensitization study.

Executive summary:

Preliminary irritation tests were performed for test chemicalAcetophenone (CAS No: 98-86-2)in guinea pigs to determine the concentration suitableforthe sensitization study[injection challenge concentration (ICC) and application challenge concentration(ACC)].

 

In the preliminary test, the guinea pigs were treated intradermally and dermally.

4 guinea pigs were (same sex) were injected intradermally in the clipped and shaved flanks with 0.1ml aliquots of a range of concentration of test substance in physiological saline. 24 hours later, the skin was examined for erythema and edema.The concentration giving slight but perceptible irritation with no oedema was selected as the injection challenge concentration (ICC).

 

Aliquots of 0.1ml using a range of concentration of the test substance in distilled water were applied in small circular areas to the shaved flanks of 4 previously untreated guinea pigs.24 hours later, the skin was examined for erythema and the highest concentration which caused no irritation was selected as the application challenge concentration (ACC).

 

As a result of preliminary studies, the concentration of Acetophenone at which no irritation was observed were 0.25% for the ICC and 20% for ACC.

 

Since the test chemical did not induce any cutaneous effects at these concentrations, the Acetophenone (CAS No: 98-86-2) was considered to be not irritating to the skin of guinea pigs.