Methyl isonicotinate

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

data from peer reviewed journals

Data source

Reference

Reference Type:

publication

Title:

Safety Assessment of Alkyl Benzoates as Used in Cosmetics

Author:

Becker LC, Bergfeld WF, Belsito DV, Hill RA, Klaassen CD, Liebler D, Marks JG Jr, Shank RC, Slaga TJ, Snyder PW, Andersen FA.

Year:

2012

Bibliographic source:

International Journal of Toxicology, November/December 2012 vol. 31 no. 6 suppl 342S-372S

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

To assess the ocular irritation potential of methyl benzoate in rabbits

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Methyl benzoate

EC Number:

202-259-7

EC Name:

Methyl benzoate

Cas Number:

93-58-3

Molecular formula:

C8H8O2

IUPAC Name:

Methylbenzoate

Test material form:

liquid

Details on test material:

- Name of test material (as cited in study report): Methylbenzoate
- Molecular formula: C8H8O2
- Molecular weight: 136.149 g/mol
- Substance type: Organic
- Physical state: Liquid
-Smiles : COC(=O)c1ccccc1
- InChI: 1S/C8H8O2/c1-10-8(9)7-5-3-2-4-6-7/h2-6H,1H3

Specific details on test material used for the study:

- Name of test material (as cited in study report): Methylbenzoate
- Molecular formula: C8H8O2
- Molecular weight: 136.149 g/mol
- Substance type: Organic
- Physical state: Liquid
-Smiles : COC(=O)c1ccccc1
- InChI: 1S/C8H8O2/c1-10-8(9)7-5-3-2-4-6-7/h2-6H,1H3

Test animals / tissue source

Species:

rabbit

Strain:

not specified

Details on test animals or tissues and environmental conditions:

no data

Test system

Vehicle:

unchanged (no vehicle)

Controls:

not specified

Amount / concentration applied:

Undiluted sample (100%)

Duration of treatment / exposure:

18 -24 hours

Observation period (in vivo):

no data

Number of animals or in vitro replicates:

5

Details on study design:

Neat (100%) methyl benzoate was applied to the center of the cornea while the lids were retracted. One minute later the lids were released. The eyes were examined 18–24 h later in strong diffuse daylight and then stained with fluorescein.

Results and discussion

In vivo

Results

Irritation parameter:

overall irritation score

Basis:

mean

Time point:

24 h

Reversibility:

not specified

Remarks on result:

positive indication of irritation

Irritant / corrosive response data:

Grade 1 irritation was observed

Applicant's summary and conclusion

Interpretation of results:

Category 2 (irritating to eyes) based on GHS criteria

Conclusions:

The ocular irritation potential of the methyl benzoate was assessed by performing rabbit eye test.
Methyl benzoate was given a grade 1 (iritis and slight internal congestion) after 24 hours of observation.
Based on this rating, methyl benzoate can be considered as an eye irritant.

Executive summary:

The ocular irritation potential of the methyl benzoate was assessed by performing rabbit eye test.

Neat (100%) methyl benzoate was applied to the center of the cornea of 5 rabbits. One minute later the lids were released. The eyes were examined 18–24 h later in strong diffuse daylight and then stained with fluorescein.Methyl benzoate was given a grade 1 (iritis and slight internal congestion) after 24 hours of observation.

Based on this rating, methyl benzoate can be considered as an eye irritant.