(S)-7-[[2-O-6-deoxy-α-L-mannopyranosyl)-β-D-glucopyranosyl]oxy]-2,3-dihydro-5-hydroxy-2-(3-hydroxy-4-methoxyphenyl)-4H-1-benzopyran-4-one

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

activated sludge respiration inhibition testing

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Justification for type of information:

REPORTING FORMAT FOR THE ANALOGUE APPROACH
See "attached justification".

Reason / purpose for cross-reference:

read-across source

Key result

Duration:

14 d

Dose descriptor:

EC50

Effect conc.:

> 105.1 mg/L

Nominal / measured:

estimated

Conc. based on:

test mat.

Basis for effect:

other: read-across from analogue.

Details on results:

The results of the read-across approach from the experimental data obtained for the supporting substance are expressed as the estimated toxicity based on molecular weights. No other adaptation is necessary.

Validity criteria fulfilled:

yes

Conclusions:

Based on the read-across approach, the target substance is not expected to be inhibitory.

Executive summary:

A toxicity control was performed during the biodegradability testing of the analogue substance naringin, according to OECD 301F (GLP study). The solutions containing 100 mg/l, of both the test and a reference item, in the mineral medium, were inoculated. The consumption of oxygen was determined from the change in pressure in the apparatus. The carbon dioxide, evolved during test item degradation, was absorbed in a solution of potassium hydroxide. The amount of oxygen taken up by the test item (corrected for uptake by blank inoculum, run in parallel) was expressed as a percentage of calculated ThOD of the test item. Under test conditions, the test item was not inhibitory, reaching a degradation of 54.1% after 14 days, and readily biodegradable. Based on the read-across approach, the target substance is not expected to be inhibitory.

Reference 2

Endpoint:

activated sludge respiration inhibition testing

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Justification for type of information:

REPORTING FORMAT FOR THE ANALOGUE APPROACH
See "attached justification".

Reason / purpose for cross-reference:

read-across source

Specific details on test material used for the study:

TEST MATERIAL
- Name of test material: neohesperidin
- IUPAC name: 5-hydroxy-2-(3-hydroxy-4-methoxyphenyl)-4-oxo-3,4-dihydro-2H-chromen-7-yl 2-O-(6-deoxyhexopyranosyl)hexopyranoside
- Molecular formula: C28H34O15
- Molecular weight: 610.5606
- Smiles notation: COc1ccc(cc1O)C2CC(=O)c3c(O)cc(OC4OC(CO)C(O)C(O)C4OC5OC(C)C(O)C(O)C5O)cc3O2
- InChl: InChI= 1/C28H34O15/c1-10-21(33)23(35)25(37)27(39-10)43-26-24(36)22(34)19(9-29)42-28(26)40-12-6-14(31)20-15(32)8-17(41-18(20)7-12)11-3-4-16(38-2)13(30)5-11/h3-7,10,17,19,21-31,33-37H,8-9H2,1-2H3

Key result

Duration:

14 d

Dose descriptor:

NOEC

Effect conc.:

> 99.67 mg/L

Nominal / measured:

estimated

Conc. based on:

test mat.

Basis for effect:

other: read-across from analogue.

Details on results:

The results of the read-across approach from the experimental data obtained for the supporting substance are expressed as the estimated toxicity based on molecular weights. No other adaptation is necessary.

Validity criteria fulfilled:

yes

Conclusions:

Based on the read-across approach, the target substance is not expected to be inhibitory.

Executive summary:

A toxicity control was performed during the biodegradability testing of the analogue substance neohesperidin dihydrochalcone, according to OECD 301F (GLP study). The solutions containing 100 mg/l, of both the test and a reference item, in the mineral medium, were inoculated. The consumption of oxygen was determined from the change in pressure in the apparatus. The carbon dioxide, evolved during test item degradation, was absorbed in a solution of potassium hydroxide. The amount of oxygen taken up by the test item (corrected for uptake by blank inoculum, run in parallel) was expressed as a percentage of calculated ThOD of the test item. Under test conditions, the test item was not inhibitory, reaching a degradation of 47.2% after 14 days, and it was readily biodegradable. Based on the read-across approach, the target substance is not expected to be inhibitory.

Description of key information

Weight of evidence: Based on the read-across approach from two analogue substances, the target substance is not inhibitory to microorganisms at 99.7 mg/L (worst-case scenario). Furthermore, the substance is readily biodegradable.

- Method according to OECD 301F, GLP study. The test item was not inhibitory to microorganisms (54.1% biodegradation in 14d) at a concentration of 100 mg/L, so the EC50 value of the test item in microorganisms is > 100 mg/L. Based on the read-across approach, the target substance is not expected to be inhibitory, with a calculated NOEC of 105.1 mg/L.

-Method according to OECD 301F, GLP study. The test item was not inhibitory to microorganisms (42.7% biodegradation in 14d) at a concentration of 100 mg/L. Therefore, the EC50 value of the test item in microorganisms is > 100 mg/L. Based on the read-across approach, the target substance is not expected to be inhibitory, with a calculated NOEC of 99.7 mg/L.

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:

99.7 mg/L

Additional information

Weight of evidence:

- A toxicity control was performed during the biodegradability testing of the analogue substance naringin, according to OECD 301F, under GLP conditions. The solutions containing 100 mg/l, of both the test and a reference item, in the mineral medium, were inoculated. The consumption of oxygen was determined from the change in pressure in the apparatus. The amount of oxygen taken up by the test item (corrected for uptake by blank inoculum, run in parallel) was expressed as a percentage of calculated ThOD of the test item. Under test conditions, the test item was not inhibitory, reaching a degradation of 54.1% after 14 days. Based on the read-across approach, the target substance is not expected to be inhibitory, with a calculated NOEC of 105.1 mg/L.

-A toxicity control was performed during the biodegradability testing of the analogue substance neohesperidin dihydrochalcone, according to OECD 301F, under GLP conditions. The solutions containing 100 mg/l, of both the test and a reference item, in the mineral medium, were inoculated. The consumption of oxygen was determined from the change in pressure in the apparatus. The amount of oxygen taken up by the test item (corrected for uptake by blank inoculum, run in parallel) was expressed as a percentage of calculated ThOD of the test item. Under test conditions, the test item was not inhibitory, reaching a degradation of 42.7% after 14 days. Based on the read-across approach, the target substance is not expected to be inhibitory, with a calculated NOEC of 99.7 mg/L.