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relative density
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From 2017-09-15 to 2017-09-15
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
according to guideline
OECD Guideline 109 (Density of Liquids and Solids)
according to guideline
EU Method A.3 (Relative Density)
GLP compliance:
yes (incl. QA statement)
Type of method:
pycnometer method
Specific details on test material used for the study:
- Batch n°: DR74271215
- Production release date: 30 November 2015
- Description: clear liquid
- Molecular formula: C13H31N3O2
- Molecular weight: 261.4 g/mol
- Expiry date: 30 December 2018
- Purity (on dry basis): 98.2%, water content: 0.05%
Key result
relative density
0.908 other: dimensionless
20 °C

Determining the Density of the Immersion Liquid

 Mass of Empty Pycnometer (g)  24.21  Volume of Pycnometer (ml)  25.7
 Mass water & Pycnometer (g)  49.84  Density of Water (g/cm³) (ρw,t)  0.998
 Mass of IL & Pycnometer (g)  49.01  Mass IL in Pycnometer (g) (m1)  24.80
 Calculated Density of IL (g/cm³)  0.965  Density of IL (kg/m³)  965

Equation 1 (Calculation for the density of water)

Mass of water / volume of pycnometer = Density of water

Equation 2 (Calculation for the density of immersion liquid)

ρIL = m1/mw x ρw,t Where : m1 is the mass of the immersion liquid; mw is the mass of water; ρw,t is the density of water at temperature t

Calculated value for the density of immersion liquid = 965 kg.m-3 @ 20°C

Determining the Density of the Sample

 Run Number  1    2
 Mass of Empty Pycnometer (g)  24.21    24.21
 Mass of Sample & Pycnometer (g)  47.55    47.52
 Mass of Sample added to empty Pycnometer (g) ms  23.34    23.31
 Mass of Sample, Pycnometer & immersion liquid  47.55    47.52
 Mass of immersion liquid added (g) m2  0.00    0.00
 Calculated Density of Sample (g/cm3)  0.909    0.907
 Density of Sample (kg/m3)  909    907
 Mean Result (kg.m-3)    908  

Equation 3 (Calculation for the density of the sample)

ρS,t = ms ρIL/ (m1 – m2)

Where: ms is the mass, in grams, of the sample;

m1 is the mass, in grams, of immersion liquid required to fill the empty pycnometer;

m2 is the mass, in grams of immersion liquid added to fill the pycnometer containing the sample;

ρIL is the density of the immersion liquid (as determined by equation 2)

The relative density of the test item was determined to be 0.908. The sample density is 908 kg/m³ at 20°C.

Description of key information

The relative density of the substance was determined using the pycnometer method and was found to be 0.908 at 20°C (density: 908 kg/m³) in a test according to OECD guideline 109 and EU Method A.3 without deviations (K1; Younis, 2017).

Key value for chemical safety assessment

Relative density at 20C:

Additional information