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Toxicological information

Acute Toxicity: dermal

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Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
secondary literature
Justification for type of information:
Data is from secondary source.

Data source

Reference
Reference Type:
secondary source
Title:
ROBUST SUMMARIES for the Diethylbenzene - Rich Streams Category
Author:
U. S. Environmental Protection Agency (EPA)
Year:
2004
Bibliographic source:
ROBUST SUMMARIES for the Diethylbenzene - Rich Streams Category, U. S. Environmental Protection Agency (EPA), 201-15249B, April 26, 2004.

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: TSCA guidelines
Principles of method if other than guideline:
Acute dermal toxicity study of Diethylbenzene (25340-17-4) in rabbits.
GLP compliance:
not specified
Test type:
other: not specified
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- IUPAC Name: Diethylbenzene
- Mol. formula: C10H14
- Molecular Weight: 134.2206 g/mole
- Smiles: CCc1ccccc1CC
- InChI: 1S/C10H14/c1-3-9-7-5-6-8-10(9)4-2/h5-8H,3-4H2,1-2H3
Specific details on test material used for the study:
- IUPAC Name: Diethylbenzene
- Mol. formula: C10H14
- Molecular Weight: 134.2206 g/mole
- Smiles: CCc1ccccc1CC
- InChI: 1S/C10H14/c1-3-9-7-5-6-8-10(9)4-2/h5-8H,3-4H2,1-2H3

Test animals

Species:
rabbit
Strain:
New Zealand White
Sex:
male/female
Details on test animals and environmental conditions:
TEST ANIMALS
- Source: No data
- Age at study initiation: at least 8 weeks old
- Weight at study initiation: The males weighed between 2.3-2.6 kg, and the females weighed between 2.6-2.7 kg.
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 60-70oF (15.5 - 21°C)
- Humidity (%):between 30-70%

Administration / exposure

Type of coverage:
other: Dermal
Vehicle:
not specified
Details on dermal exposure:
not specified
Duration of exposure:
not specified
Doses:
5000 mg/kg
No. of animals per sex per dose:
Total = 10 (Male/Female)
Control animals:
not specified
Details on study design:
- Duration of observation period following administration: 14 days
- Other examinations performed: Animals were observed for clinical examination and body weight (loss/gain).
Statistics:
FIFRA method was used.

Results and discussion

Preliminary study:
not specified
Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
>= 5 000 mg/kg bw
Based on:
test mat.
Remarks on result:
other: No Mortality observed
Mortality:
No Mortality observed at 5000 mg/kg bw
Clinical signs:
Fissuring exhibited at a small portion of the dose site, in 1 animal, no severe dermal effect were seen. Decreased food consumption was exhibited by all 10 animals on the day after dosing; by 4 animals on Day 4; and by 1 animal on Day 10.
Body weight:
All animals exhibited body weight losses or no weight change at Day 7, but most gained weight between Days 7 and 14.
Gross pathology:
not specified
Other findings:
not specified

Applicant's summary and conclusion

Interpretation of results:
Category 5 based on GHS criteria
Conclusions:
The LD50 was considered to be = >5000 mg/kg bw, when male and female New Zealand White rabbits were treated with Diethylbenzene (25340-17-4) by dermal application.
Executive summary:

Acute Dermal toxicity study was conducted according to TSCA guideline in 10 Male and Female New Zealand White rabbits using Diethylbenzene at the concentration of 5000 mg/kg bw. At start of the experiment, animals were at least 8 weeks old. The males weighed between 2.3-2.6 kg, and the females weighed between 2.6-2.7 kg. Room temperature was 60-70°F, and relative humidity was between 30-70% during the study. Animals were observed for 14 days post dose.Animals were observed for clinical examination and body weight (loss/gain). FIFRA method was used.No Mortality observed at5000 mg/kg bw. Fissuring exhibited at a small portion of the dose site, in 1 animal, no severe dermal effect were seen. Decreased food consumption was exhibited by all 10 animals on the day after dosing; by 4 animals on Day 4; and by 1 animal on Day 10.All animals exhibited body weight losses or no weight change at Day 7, but most gained weight between Days 7 and 14. Therefore, LD50 was considered to be = >5000 mg/kg bw, when male and female New Zealand White rabbits were treated with Diethylbenzene (25340-17-4) by dermal application.