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EC number: 606-396-7 | CAS number: 198904-86-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 3rd to 23rd December 1999
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Off-white powder, 99.15% purity
- Analytical monitoring:
- yes
- Vehicle:
- yes
- Remarks:
- dechlorinated tap water
- Details on test solutions:
- The results of a preliminary solubility trial showed that BMS 233101-01 was insufficiently soluble in water to allow the preparation of a suitable stock solution, so appropriate weights were added directly to test beakers. Dechlorinated tap water (284 ml) was added and the mixtures were treated with ultrasound for 10 minutes before the addition of remaining components of the mixture.
The prepared mixtures were aerated for three hours using a Pasteur pipette connected to a laboratory supply of oil-free compressed air
A concentrated solution of 3,5-DCP (500 mg/l) was prepared by dissolving 0.5 g in 10 ml of in sodium hydroxide and diluting to approximately 30 ml with ultrapure water. Sulphuric acid (IN) was added to the point of incipient precipitation and the solution made up to a final volume of one litre with ultrapure water. The pH of this solution was then measured.
Nominal concentrations of 3.0, 10.0 and 32.0 mg/l were prepared by dilution of this concentrated solution. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- A sample of activated sludge was obtained the day before the start of the test from Oakley Sewage Treatment Works, which treats predominantly domestic waste. In the laboratory, the sample was maintained under aerobic conditions until required.
The concentration of suspended solids in a homogenised sample was determined on the day of collection and immediately before the start of the test.
On the day of collection, aliquots (25 ml) of the activated sludge were filtered through dried and preweighed Whatman GFC filter papers which were then dried again at approximately 105oC for at least one hour, allowed to cool in a desiccator and reweighed. The mixed liquor suspended solids
(MLSS) content of the activated sludge was then calculated. Synthetic sewage (50 ml/1) was added and the mixture aerated overnight.
On the day of the test, the MLSS content of the sludge was determined and adjusted to 4 g/1 by the addition of dechlorinated tap water. The pH of the sludge was also measured. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Test temperature:
- 18.7-21 C during the test
- pH:
- Before addition of sludge: 7.7 -7.8
After 3 hours of exposure: 8.2 - 8.4 (includes conditions for reference item) - Nominal and measured concentrations:
- Nominal concentrations: 100mg
- Details on test conditions:
- TEST SYSTEM
- Test vessel: all-glass, 1 Litre, open bottles/vessels, fill volume: 500 mL
- Aeration: The aeration was adjusted in such a way that the dissolved oxygen concentration at the st
art was above 60-70% saturation (60% of air saturation is > 5 mg/L at 20°C) and to maintain the slu
dge flocs in suspension. Clean, oil-free air was used for air supply.
- No. of vessels per concentration (replicates): 5 - Reference substance (positive control):
- yes
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- Any observations that might cause a difference between measured and nominal values: no
- Results with reference substance (positive control):
- Results with reference substance valid? yes
- Validity criteria fulfilled:
- yes
- Conclusions:
- Validity criteria fulfilled yes
1. Mean control oxygen uptake rate > 20 mg O2/g activated sludge in an hour (29 mg O2/g).
2. CV of oxygen uptake in controls < 30% (11%) 3. EC50 of 3,5-dichlorophenol was in the accepted range of 2-25 mg/L for total respiration (estimated value: 12 mg/L).
BMS 233101-01 had no inhibitory effect on the respiration rate of activated sludge at any of the concentrations employed in the test. The ECjo, ECso and EQo of the test substance could not, therefore, be calculated but these must be greater than 100 mg/1, the highest level tested.
The three-hour EC5o for 3,5-DCP (13.2 mg/1) fulfilled the validity criterion relating to sensitivity to inhibition (acceptable EC50 range 5 to 30 mg/1), and that relating to the respiration rates in the control (variation not greater than 15%) was also satisfied. - Executive summary:
The effect of BMS 233101-01 on the respiration rate of activated sludge was assessed by the methods detailed in EC Directive 88/302, 'Biodegradation - Activated Sludge Respiration Inhibition test' and
OECD Test Guideline 209, 'Activated Sludge, Respiration Inhibition test'.
Samples of activated sludge (suspended solids 1.6 g/1), fed with synthetic sewage, were exposed to the test substance at nominal concentrations of 1, 10 and 100 mg/1 for three hours. Single mixtures
were prepared at 1 and 10 mg/1 and the highest level was prepared in triplicate. Their rates of oxygen consumption were determined and compared with those of controls, containing activated sludge and
synthetic sewage alone, which were established at the beginning and end of the culture series. The reference inhibitor 3,5-dichlorophenol (3,5-DCP) was employed at 3.0, 10.0 and 32.0 mg/1, as a
positive control.
The specific respiration rate of the control culture established at the end of the test series (26.8 mgCVg/h) was 104% of the rate of that established at the start (25.9 mgCVg/h). The three-hour
50% effect concentration (ECso) for 3,5-DCP was calculated to be 13.2 mg/1 (95% confidence limits 10.4 - 17.3 mg/1). These results show that the test was valid and that the sample of activated sludge
employed was sensitive to inhibition. BMS 233101-01 had no inhibitory effect on the respiration rate of activated sludge at any of the concentrations employed in the test. The EC20, EC50 and EC80 of the test substance could not, therefore, be calculated but these must be greater than 100 mg/1, the highest level tested
Reference
Sludge respiration rates were progressively reduced in the presence of increasing concentrations of 3,5-DCP. The three-hour 50% effect concentration (EC50) for 3,5-DCP was calculated by the Moving
Average method to be 13.2 mg/l (95% confidence limits 10.4 -17.3 mg/l). The specific respiration rate of the control culture established at the end of the test (26.8 mg02/g/h)
was 104% of the rate of that established at the start (25.9 mg02/g/h). These results show that the test was valid and that the sample of activated sludge employed was
sensitive to inhibition. BMS 233101-01 had no inhibitory effect on the respiration rate of activated sludge at any of the concentrations employed in the test. The EC20, EC50 and EC80 of the test substance could not, therefore, be calculated but these must be greater than 100 mg/l, the highest level tested.
Description of key information
BMS 233101-01 had no inhibitory effect on the respiration rate of activated sludge at any of the concentrations employed in the test. The EC20, EC50 and EC80 of the test substance could not, therefore, be calculated but these must be greater than 100 mg/1, the highest level tested
Key value for chemical safety assessment
- EC50 for microorganisms:
- 100 mg/L
Additional information
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