Reaction mass of ammonium dodecyl sulfate, ammonium tetradecyl sulfate and ammonium alkoxy-alkyl sulfate

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

25 Feb - 28 Feb 1997

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Refer to the Analogue Approach Justification document provided in IUCLID6 Section 13.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1997

Report date:

1997

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.3 (Algal Inhibition test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Sulfuric acid, mono-C12-18-alkyl esters, sodium salts

EC Number:

273-257-1

EC Name:

Sulfuric acid, mono-C12-18-alkyl esters, sodium salts

Cas Number:

68955-19-1

IUPAC Name:

sodium pentadecyl sulfate

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 0, 1, 3, 10, 30, 100 mg/L

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 1.54 g/L test substance in VE water (stock solution)

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM
- Strain: CHODAT (86.81 SAG)
- Source (laboratory, culture collection): Institut für Wasser-, Boden- und Lufthygiene, Berlin, Germany (originally); in-house culture
- Age of inoculum (at test initiation): 3 days

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Post exposure observation period:

not relevant

Test conditions

Hardness:

no data

Test temperature:

23.5-23.8 °C

pH:

Begin: 7.8-8.0
End: 7.3-9.4

Dissolved oxygen:

no data

Salinity:

not relevant

Nominal and measured concentrations:

Nominal: 0, 1.5, 3, 6, 12, 24, 48 and 96 mg/L
Measured: >80% at all concentrations, nominal values used

Details on test conditions:

TEST SYSTEM
- Test vessel: sterile Erlenmeyer flasks
- Initial cell density: 20000 cells/mL
- Control end cells density: 1,160,000 cells/mL

GROWTH MEDIUM
- Standard medium used: yes


OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Photoperiod: continuous illumination
- Light intensity and quality: 8000 lux (white)


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : cell number and cell growth
- Determination of cell concentrations: spectrophotometer at 685 nm

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

20 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

7.6 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

growth rate

Effect concentrations 3

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

3 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

growth rate

Effect concentrations 4

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

6.4 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

biomass

Effect concentrations 5

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

14 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

biomass

Details on results:

- Exponential growth in the control (for algal test): yes (20,000 to 1,160,000 cells/mL)
- Observation of abnormalities (for algal test): no

Reported statistics and error estimates:

Probit analyis (Cavalli-Sforza 1972)