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Short-term toxicity to aquatic invertebrates

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short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study. Pre dates OECD Guideline
according to
other: ASTM Standard E 729-80
GLP compliance:
not specified
Analytical monitoring:
Test organisms (species):
Daphnia magna
Details on test organisms:
- Common name: Daphnid
- Source: Brood stock
- Age at study initiation (mean and range, SD): < 24h
- Method of breeding: The brood stock was cultured and maintained in aerated (90-105% saturation) 2.91 - liter animal jars. A 2.0-liter open-ended tube with 1.0 mm nylon bolting cloth affixed to the bottom was contained within each jar to facilitate harvesting first instars. Th screened end of the tube was held approximately 1.5 cm off the bottom by two Pyrex triangles placed one on top of the other. Each tube contained 25 adult daphnids. When the tube was lifted, the adults remained on the screen while the first instars passed into the beaker for harvesting. Each brood stock animal jar contained the appropriate amount of food and water made up to a 2.0 liter volume. The brood stock food, water and glass receptacles were changed 3 time per week. The brood stock was kept in an environmental chamber set at 20 +-1°C and a photoperiod of 16h light : 8h dark. Twenty-four hours before testing, mutiparous females were isolated. The neonates produced by these adults were removed and used for testing within 24h.
- Feeding during test : no feeding during test
Test type:
Water media type:
Limit test:
Total exposure duration:
48 h
148 +- 7 mg/L as CaCO3
Test temperature:
20.1 - 20.7
6.7 - 8.1
Dissolved oxygen:
> 60% saturation
Nominal and measured concentrations:
The nominal concentrations were : 54, 91, 151, 252, 420 and 700 mg/l as boron
Details on test conditions:
- Test vessel:
- Material, size, headspace, fill volume: 250 ml beakers to which 200 ml of the appropriate amount of test material and water was added.
- Aeration: no aeration during test
- No. of organisms per vessel: 10 neonates

- Source/preparation of dilution water: Lake Huron water was used to culture daphnids and as the test dilution water. This water was carbon-filtered, UV-irradiated and adjusted to a hardness of approximately 170 mg/L as (CaCO3) with CaCl2. The water was then autoclaved at 120°C and 124.1 kPa for 35 min.
- Alkalinity:58 +- 5 mg/L as CaCO3
- Conductivity: 290 +- 21 µmhos/cm

- Photoperiod: 16h light : 8 h dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality was recorded after 24 and 48h of exposure
48 h
Dose descriptor:
Effect conc.:
133 mg/L
Nominal / measured:
Conc. based on:
Basis for effect:
Remarks on result:
other: 115-153 mg/L
Details on results:
- Mortality of control: 7%
Reported statistics and error estimates:
The LC50 and 95% confidence intervals were determined. The LC50 values were based on nominal concentrations. Finney's method of probit analysis or the moving angle method was used to calculate the LC50 values.
Duration Endpoint Effect conc. Nominal/Measured Conc. based on Basis for effect Remarks (e.g. 95% CL)
48 h LC50 133 mg/L nominal element mortality
48 h LC0 54 mg/L nominal element mortality
48 h LC100 420 mg/L nominal element mortality

Description of key information

Key value for chemical safety assessment

Additional information

Short term effects on aquatic invertebrates ranged from 45 to 1376 mg B/L.

These data support a conclusion that borates should not be classified as dangerous for the environment.