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EC number: 272-729-4 | CAS number: 68910-05-4 A complex residuum from the fractionation of the reaction products of 2-aminoethanol with ammonia to remove piperazine. It may contain such compounds as 2-[(2-aminoethyl)amino]ethanol, (aminoethyl)piperazine, (hydroxyethyl)piperazine.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
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- Nanomaterial pour density
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- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
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- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Glp guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Ethanol, 2-amino-, reaction products with ammonia, by-products from
- EC Number:
- 272-729-4
- EC Name:
- Ethanol, 2-amino-, reaction products with ammonia, by-products from
- Cas Number:
- 68910-05-4
- Molecular formula:
- Unspecified
- IUPAC Name:
- Ethanol, 2-amino-, reaction products with ammonia, by-products from
- Details on test material:
- - Name of test material (as cited in study report):Diethylentriamine R
- Physical state: liquid
- Analytical purity: The identity of the test substance was confirmed...........
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Details on mammalian cell type (if applicable):
- - Properly maintained: yes
- Periodically "cleansed" against high spontaneous background: yes
- Species / strain / cell type:
- E. coli WP2 uvr A
- Details on mammalian cell type (if applicable):
- - Properly maintained: yes
- Periodically "cleansed" against high spontaneous background: yes
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9-mix prepared form sprague Dawlay rat livers after Aroclor 1254 activation
- Test concentrations with justification for top dose:
- 0, 20, 100, 500, 2500, 5000 µg/plate (SPT)
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other: see details
- Details on test system and experimental conditions:
-
Standard Plate Test
Salmonella typhimurium
Test tubes containing 2-mL portions of soft agar (overlay agar), which consists of 100 mL agar (0.8% [w/v] agar + 0.6% [w/v] NaCl) and 10 mL amino acid solution (minimal amino acid solution for the determination of mutants: 0.5 mM histidine + 0.5 mM biotin) are kept in a water bath at about 42 - 45°C, and the remaining components are added in the following order:
0.1 mL test solution or vehicle (negative control)
0.1 mL fresh bacterial culture
0.5 mL S9 mix (with metabolic activation)
or
0.5 mL phosphate buffer (without metabolic activation)
After mixing, the samples are poured onto Vogel-Bonner agar plates (minimal glucose agar plates) within approx. 30 seconds.
Composition of the minimal glucose agar:
980 mL purified water
20 mL Vogel-Bonner E medium
15 g Difco bacto agar
20 g D-glucose, monohydrate.
After incubation at 37°C for 48 – 72 hours in the dark, the bacterial colonies (his+ revertants) are counted.
Escherichia coli
Test tubes containing 2-mL portions of soft agar (overlay agar), which consists of 100 mL agar (0.8% [w/v] agar + 0.6% [w/v] NaCl) and 10 mL amino acid solution (minimal amino acid solution for the determination of mutants: 0.5 mM tryptophan) are kept in a water bath at about 42 - 45°C, and the remaining components are added in the following order:
0.1 mL test solution or vehicle (negative control)
0.1 mL fresh bacterial culture
0.5 mL S9 mix (with metabolic activation)
Or
0.5 mL phosphate buffer (without metabolic activation)
After mixing, the samples are poured onto minimal agar plates within approx. 30 seconds.
The composition of the minimal agar (SA1 selective agar) is based on the description of Green, M.H.L. and Muriel, W.J. ( 5), with the exception of the amino acid solution, which has previously been added to the soft agar:
300 mL solution B (agar)
100 mL solution A (saline solution)
8 mL solution C (glucose solution)
10 mL solution D (casein solution)
After incubation at 37°C for 48 - 72 hours in the dark, the bacterial colonies (trp+ revertants) are counted.
In each experiment 3 test plates per dose or per control were used.
Positive controls:
2-Aminoanthracene: 2.5 µg/plate for strains: TA 1535, TA 100, TA 1537, TA 98 ( with S9 mix)
60 µg/plate for strain: E. Coli WP2 uvrA ( with S9 mix)
N-methyl-N`-nito-N-nitrosoguanidine : 5 µg/plate for strains: TA 1535, TA 100 (without S9 mix)
4-nitro-o-phenylenediamine: 10 µg/plate for strain: TA 98 (without S9 mix)
9-aminoacridine: 100 µg/plate for strain: TA 1537 (without S9 mix)
4-nitroquinoline-N-oxide: 5 µg/plate for strain: E.coli WP2 uvrA (without S9 mix)
Dissolved in DMSO.
The titer is generally determined only in the experimental parts with S9 mix both for the negative controls and for the two highest doses in all experiments.
- Evaluation criteria:
- Positive results:
A dose-related and reproducible increase in the number of revertant colonies, i.e. about doubling of the spontaneous mutation rate in at least one tester strain either without S9 mix or after adding a metabolizing system.
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Mutagenicity
Strain |
Metabolic activation |
Experiment No. |
Concentration [µg/plate] |
Replicates |
Max. factor of revertants |
TA 1535 |
No No |
1 2 |
20-5000 20-5000 |
3 3 |
No relevant increase in the numbers of his+ or trp+ revertans |
TA 100 |
No No |
1 2 |
20-5000 20-5000 |
3 3 |
4.2 4.3 |
TA 1537 |
No No |
1 2 |
20-5000 20-5000 |
3 3 |
2.4 2.3 |
TA 98 |
No No |
1 2 |
20-5000 20-5000 |
3 3 |
3.0 3.5 |
E.coli WP2 uvrA |
No No |
1 2 |
20-5000 20-5000 |
3 3 |
8.7 9.6 |
TA 1535 |
Yes Yes |
1 2 |
20-5000 20-5000 |
3 3 |
No relevant increase in the numbers of his+ or trp+ revertans |
TA 100 |
Yes Yes |
1 2 |
20-5000 20-5000 |
3 3 |
2.8 4.0 |
TA 1537 |
Yes Yes |
1 2 |
20-5000 20-5000 |
3 3 |
1.6 2.5 |
TA 98 |
Yes Yes |
1 2 |
20-5000 20-5000 |
3 3 |
2.7 3.4 |
E.coli WP2 uvrA |
Yes Yes |
1 2 |
20-5000 20-5000 |
3 3 |
4.0 5.7 |
Applicant's summary and conclusion
- Conclusions:
- Thus, under the experimental conditions chosen here, it is concluded that Diethylen-triamine R is a mutagenic test substance in the bacterial reverse mutation test in the absence and the presence of metabolic activation.
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