Registration Dossier

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
2000
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: guideline study without detailed description

Data source

Reference
Reference Type:
publication
Title:
QSAR study on the toxicity of substitued benzenes to the algae (Scenedesmus obliquus)
Author:
Lu G.H., Yuan X., Zhao Y.H.
Year:
2001
Bibliographic source:
Chemosphere, vol. 44, p. 437-440, 2001

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
not specified
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
not specified

Test solutions

Vehicle:
not specified

Test organisms

Test organisms (species):
other: Scenedesmus obliquus

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Test temperature:
20±1°C
pH:
7.2±0.2
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 250 ml Erlenmeyer flasks (fill in 60 ml)
- Initial cells density: cca 10000 cells/ml


GROWTH MEDIUM
- Detailed composition if non-standard medium was used:
NH4Cl 15 mg/l
MgCl2 . 6H2O 12 mg/l
CaCl2 . 2H2O 18 mg/l
MgSO4 . 7H2O 15 mg/l
KH2PO4 1.6 mg/l
NaHCO3 50 mg/l
Na2EDTA . 2H2O 0.1 mg/l
FeCl3 . 6H2O 0.08 mg/l
H3BO3 0.185 mg/l
MnCl2 . 4H2O 0.415 mg/l
ZnCl2 0.003 mg/l
CoCl2 . 6H2O 0.0015 mg/l
Na2MoO4 . 2H2O 0.007 mg/l
CuCl2 . 2H2O 0.00001 mg/l


OTHER TEST CONDITIONS
- Photoperiod: continuous light
- Light intensity and quality: cca 400 lux (white fluorescent lamp)


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: electron microscope (400×)
Reference substance (positive control):
not specified

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
0.15 mol/L
Nominal / measured:
not specified
Conc. based on:
not specified
Basis for effect:
growth rate

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified