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Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
April 2013
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2013
Report date:
2013

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
Deviations:
no
Remarks:
An optional toxicity control as suggested by OECD guideline 301F was not performed. The validity of the study is not adversely affected.
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
Methyl oct-2-ynoate
EC Number:
203-836-6
EC Name:
Methyl oct-2-ynoate
Cas Number:
111-12-6
Molecular formula:
C9H14O2
IUPAC Name:
methyl oct-2-ynoate
Test material form:
liquid

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Duration of test (contact time):
30 d
Initial test substance concentrationopen allclose all
Initial conc.:
30 mg/L
Based on:
test mat.
Remarks:
Nominal concentration
Initial conc.:
100 mg/L
Based on:
other: reference substance
Remarks:
Nominal concentration
Details on study design:
A measured volume of inoculated mineral medium, containing a known concentration of test substance as the nominal sole source of organic carbon, is stirred in a closed flask at a constant temperature (± 1°C) for up to 28 days. Evolved carbon dioxide is absorbed in sodium hydroxide pellets. The consumption of oxygen is determined by measuring the pressure drop in the respirometer flask. The Biological Oxygen Demand (BOD), amount of oxygen taken up by the microbial population during biodegradation of the test chemical (corrected for uptake by blank inoculum, run in parallel) is expressed as a percentage of ThOD (Theoretical Oxygen Demand, calculated from the elemental composition, assuming that carbon is oxidized to carbon dioxide, hydrogen to water and nitrogen to ammonium, nitrite or nitrate).
Reference substance
Reference substance:
other: Sodium benzoate

Results and discussion

% Degradation
Key result
Parameter:
% degradation (O2 consumption)
Value:
80
Sampling time:
28 d

BOD5 / COD results

Results with reference substance:
Degradation of the reference substance, Sodium Benzoate, attained 72% by Day 7 and 84% by Day 14.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Executive summary:

Folione undergoes 80% biodegradation after 28 days in the test conditions. The 10 -day window criterion is also fulfilled (29% biodegradation on day 5 and 71% on day 15).

Folione did not inhibit the intrinsic respiration of the inoculum at the test concentration and was therefore considered to be non-toxic to the inoculum at the test concentration.

Therefore, Folione should be regarded as readily biodegradable according to this test.

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