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EC number: 230-660-7 | CAS number: 7250-67-1
Endpoint summary
Administrative data
Description of key information
Biodegradation in water
Biodegradability of 1-(2-chloroethyl)pyrrolidine hydrochloride (CAS no. 7250 -67 -1) is predicted using OECD QSAR toolbox version 3.3 with logKow as the primary descriptor (2017). Test substance undergoes 37.5% degradation by BOD parameter in 28 days.Thus, based on percentage degradation, the test chemical 1-(2-chloroethyl)pyrrolidine hydrochloride was estimated to be not readily biodegradable in water.
Additional information
Biodegradation in water
Predicted data for the target compound1-(2-chloroethyl)pyrrolidine hydrochloride (CAS No. 7250-67-1) and various study for its read across substance were reviewed for the biodegradation end point which are summarized as below:
For target chemical 1-(2-chloroethyl)pyrrolidine hydrochloride (CAS no. 7250 -67 -1), biodegradability is predicted using OECD QSAR toolbox version 3.3 with logKow as the primary descriptor (2017). Test substance undergoes 37.5% degradation by BOD parameter in 28 days. Thus, based on percentage degradation, the test chemical 1-(2-chloroethyl)pyrrolidine hydrochloride was estimated to be not readily biodegradable in water.
In a weight of evidence study from peer reviewed journal (Kenneth S et. al, 1974) for the read across chemical1,2-Dichloroethane (CAS no. 107-06-2), biodegradation experiment was conducted for 20 days for evaluating the percentage biodegradability of read across substance 1,2-Dichloroethane. Initial test substance conc. used in the study was 3, 7 and 10 mg/l, respectively. Domestic wastewater was used as a test inoculum. The BOD bottles were half filled with aerated dilution water containing the specified minerals and buffer. Small aliquots of the test chemicals were added to these bottles from 0.1% stock solutions yielding conc. of 3, 7 and 10 mg/l. At least two of these concentrations were tested in duplicate. These concentrations gave a potential oxygen demand of 3 to 30 mg/l over the 20 days duration of test. Dissolved oxygen was monitored periodically in the individual bottles through the use of a commercial DO meter filled with an agitated probe. The bottles were opened for sampling and DO measurements about five times during the course of the 20 day test. The percentage degradation of read across substance was determined to be 0 and 18% degradation in 5 and 10 days, respectively. Thus, based on percentage degradation, 1,2 -Dichloroethane is considered to be not readily biodegradable in nature.
Another biodegradation study for the same read across substance 1,2 -Dichloroethane (CAS no. 107-06-2) was conducted for 28 days for evaluating the percentage biodegradability of substance 1,2 –Dichloroethane(Milena Eleonore Lapertot, Cesar Pulgarin, 2006). The study was performed according to Manometric respirometry test. Initial test substance conc. used in the study was 50 mg/l. Activated sludge was used as a test inoculum. The inoculum—activated sludge—came from a secondary effluent of the treatment plant of Morges (Switzerland). The fresh sample was centrifuged at 10000 RPM during 7 min at 20°C, and washed once with mineral medium. The average concentration of biomass was 25 mg of suspended solids per liter of mineral nutrient medium. The manometric respirometry test (Directive 92/69/EEC) were executed using a Hg free WTW 2000 Oxitop©unit thermostated at 20°C. Experiments were carried out according to official protocols. This equipment facilitated the manipulation and minimized the quantity of vessels required. Indeed when O2 is consumed, the CO2 produced is trapped by sodium hydroxide pellets and a depression in the bottle is automatically recorded. The volume of the solutions poured in the bottles was determined according to the theoretical oxygen demand without nitrification (ThODNH3 ).28 d test was duplicated. The chemical analyses were replicated thrice. Standard deviation was less than 5%.The percentage degradation of read across substance was determined to be 25% by GC or theoretical oxygen uptake parameter in 26 days. Thus, based on percentage degradation, Dichloroethane is considered to be not readily biodegradable in nature.
In a weight of evidence study, biodegradation experiment was conducted for 28 days for evaluating the percentage biodegradability of read across substance Dichloromethane (CAS no. 75 -09 -2) (Milena Eleonore Lapertot, Cesar Pulgarin, 2006). The study was performed according to Manometric respirometry test. Initial test substance conc. used in the study was 50 mg/l. Activated sludge was used as a test inoculum. The inoculum—activated sludge—came from a secondary effluent of the treatment plant of Morges (Switzerland). The fresh sample was centrifuged at 10000 RPM during 7 min at 20°C, and washed once with mineral medium. The average concentration of biomass was 25 mg of suspended solids per liter of mineral nutrient medium. The manometric respirometry test (Directive 92/69/EEC) were executed using a Hg free WTW 2000 Oxitop©unit thermostated at 20°C. Experiments were carried out according to official protocols. This equipment facilitated the manipulation and minimized the quantity of vessels required. Indeed when O2 is consumed, the CO2 produced is trapped by sodium hydroxide pellets and a depression in the bottle is automatically recorded. The volume of the solutions poured in the bottles was determined according to the theoretical oxygen demand without nitrification (ThODNH3).28 d test was duplicated. The chemical analyses were replicated thrice. Standard deviation was less than 5%. The percentage degradation of read across substance was determined to be 0% by chromatography or theoretical oxygen uptake parameter in 28 days. Thus, based on percentage degradation, Dichloromethane is considered to be not readily biodegradable in nature.
For the same read across chemical Dichloromethane (CAS no. 75 -09 -2) from authoritative database (J-CHECK, 2016), biodegradation study was conducted for 28 days for evaluating the percentage biodegradability of read across substance Dichloromethane. Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. The percentage degradation of read across substance was determined to be 13% and 1% by BOD and GC parameter in 28 days. Thus, based on percentage degradation, Dichloromethane is considered to be not readily biodegradable in nature.
On the basis of above results for target chemical 1-(2-chloroethyl)pyrrolidine hydrochloride (fromOECD QSAR toolbox version 3.3, 2017) and for its read across substance (from peer reviewed journals and authoritative database J-CHECK, 2016), it can be concluded that the test substance 1-(2-chloroethyl)pyrrolidine hydrochloride can be expected to be not readily biodegradable in nature.
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