N-(2-chloroethyl)pyrrolidine hydrochloride

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

26-06-2017 to 08-07-2017

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed according to the guideline.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Principles of method if other than guideline:

Study was conducted to access the effect of test chemical on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous stirring of 1 hour for achieving test concentrations of 100 mg/L, respectively.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name:Zebra Fish
- Strain:Danio rerio
- Source: Unique Aqua Fish Corner
- Length at study initiation (length definition, mean, range and SD): average 1.78 cm
- Weight at study initiation (mean and range, SD): average 0.479g
-Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
-Duration of housing: 15 days
-Photoperiod: 16 hours light and 8 hours dark
-Diet : Standard brand feed, once daily
-Acclimatization: seven days
-feeding frequency: Once in a day

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

150.5 mg of CaCO3

Test temperature:

23 °C

pH:

7.09 -7.7

Dissolved oxygen:

7.7 (mg/l)

Nominal and measured concentrations:

100mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Plastic aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours dark

Reference substance (positive control):

not specified

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: mortality was not observed

Sublethal observations / clinical signs:

8.1. Mortality and symptoms:

Nominal Concentration (mg/l)	Number of fish		Mortality (cumulated) after
			3h		6h		24h		48h		72h		96h
			V1	V2	V1	V2	V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	-	-	-	-	-	-	-	-	-	-	-	-
100	4	4	-	-	-	-	-	-	-	-	-	-	-	-

Visible Symptoms:

Nominal Concentration (mg/l)

Number of fish

symptom (cumulated) after

3h

6h

24h

48h

72h

96h

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

100

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS31S1

NS4

M4

M4

M4

M4

Explanation of symptoms:

A: apathy

N: Narcotic-like state

C: Swimming near water surface

M: Slow movements compared to control

NS: No abnormal symptoms

S: Surfacing behaviour

L: Loss of equilibrium

SM: Shimming movement

H1: Hyperactivity

H2: Hypo activity

B: Bending behavior

F: Fin rot

IS: Invert Swimming

SB: Swimming behaviour

RF: Respiratory Function

P: Pigmentation

pH and oxygen content:

                            

Nominal Concentration (mg/l)	Number of fish		pH readings after
			24 h		48 h		72 h		96 h
			V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	7.5	7.5	8.0	8.1	8.0	8.0	8.1	8.1
100	4	4	7.4	7.4	7.7	7.6	7.9	7.9	8.1	8.1

 

 

Nominal Concentration (mg/l)	Number of fish		Oxygen content (mg/L) after
			24 h		48 h		72 h		96 h
			V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	6.0	6.0	5.9	5.9	5.9	5.9	5.8	5.8
100	4	4	6.2	6.2	5.8	5.8	5.7	5.7	5.6	5.6

 

8.3. Test temperature

Nominal Concentration (mg/l)	Number of fish		Temperature (°C) after
			24 h		48 h		72 h		96 h
			V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	23.9	23.9	22.7	22.7	22.3	22.3	22.4	22.4
100	4	4	23.8	23.8	22.3	22.3	22.7	22.7	22.8	22.8

Validity criteria fulfilled:

yes

Conclusions:

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for CAS No. on Zebra Fish Danio rerio was determine to be > 100 mg/L.

Executive summary:

Study was conducted to assess the effect of test chemical on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).

The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous stirring of 1 hour for achieving test concentrations of 100 mg/L, respectively and Zebra Fish Danio rerio were exposed to these concentration for 96 hours. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203.

After 96 hours of exposure of test chemical at of 100mg/L, no mortality was observed. Therefore the LC50 was determined to be >100mg/L. Based on the LC50, the test chemical can be considered as non-toxic and can be concluded as not classified as per the CLP classification criteria.

Description of key information

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for CAS No. on Zebra Fish Danio rerio was determine to be > 100 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L

Additional information

Various experimental data were reviewed for to assess the toxic effects of test compound which are summarized as below:

 

Study was conducted to assess the effect of test chemical on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).

The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous stirring of 1 hour for achieving test concentrations of 100 mg/L, respectively and Zebra Fish Danio rerio were exposed to these concentration for 96 hours. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203.

After 96 hours of exposure of test chemical at of 100mg/L, no mortality was observed. Therefore the LC50 was determined to be >100mg/L.

 

The above result was supported by another study. Ninety-six-hour flow-through exposures using 31-d-old juvenile fathead minnows were conducted on 617 chemicals including our target chemical. Tests were conducted using Lake Superior water at 25 (+-) 18C. Aqueous toxicant concentrations were measured in test with quality assurance criteria requiring 80% agreement between duplicate samples and 90 to 110% spike recovery. Flow-through exposures were conducted using cycling proportional, modified Benoit or electronic diluters.

Median lethal concentrations (LC50s) were calculated using the Trimmed Spearman–Karber Method, with 95% confidence intervals being calculated when possible.

Results show 50% mortality after 96 hours at dose concentration 153 mg/l. Therefore, LC50 was considered to be 153 mg/L when exposed to test chemical.

 

Based on the LC50 values, the test chemical can be considered as non-toxic and can be concluded as not classified as per the CLP classification criteria.