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Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
other information
Study period:
26. Sep to 26. Oct 2000
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: well reported GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2001
Report date:
2001

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
3-oxopregn-4-ene-21,17α-carbolactone
EC Number:
213-552-4
EC Name:
3-oxopregn-4-ene-21,17α-carbolactone
Cas Number:
976-70-5
Molecular formula:
C22H30O3
IUPAC Name:
(1R,3aS,3bR,9aR,9bS,11aS)-9a,11a-dimethyl-2,3,3a,3b,4,5,7,8,9,9a,9b,10,11,11a-tetradecahydrospiro[cyclopenta[a]phenanthrene-1,2'-oxolane]-5',7-dione
Details on test material:
- Name of test material (as cited in study report): aldona (ZK 57564)
- Analytical purity: 95.5%
- Lot/batch No.: 59035042
- Expiration date of the lot/batch: Aug 2002

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Duration of test (contact time):
<= 28 d
Initial test substance concentration
Initial conc.:
ca. 8.7 mg/L
Based on:
other: concentration of dissolved oxygen

Results and discussion

% Degradation
Parameter:
% degradation (O2 consumption)
Value:
ca. 30
Sampling time:
11 d
Details on results:
The test compound reached a plateau of degradation on day 11 with 30% degradation, but remained there until the end of the incubation period. The reference material exceeded 60% degradation on day 4. The toxicity control did not indicate an inhibitory effect of aldona

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
The reference material exceeded 60% degradation on day 4
Executive summary:

The aerobic biodegradation of aldona was studied in a closed bottle test according to the test guideline OECD 301D. The test compound was incubated at a concentration of 3.2 mg/L over a period of 28 days. Additionally, a reference substance (sodium acetate) and a toxicity control including the test compound and sodium acetate was used. The test compound reached a plateau of degradation on day 11 with 30% degradation, but remained there until the end of the incubation period. The reference material exceeded 60% degradation on day 4, fulfilling the validity criteria. The toxicity control did not indicate an inhibitory effect of aldona. Thus, aldona is not ready biodegradable.