3-oxopregn-4-ene-21,17α-carbolactone

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

other information

Study period:

26. Sep to 26. Oct 2000

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: well reported GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

3-oxopregn-4-ene-21,17α-carbolactone

EC Number:

213-552-4

EC Name:

3-oxopregn-4-ene-21,17α-carbolactone

Cas Number:

976-70-5

Molecular formula:

C22H30O3

IUPAC Name:

(1R,3aS,3bR,9aR,9bS,11aS)-9a,11a-dimethyl-2,3,3a,3b,4,5,7,8,9,9a,9b,10,11,11a-tetradecahydrospiro[cyclopenta[a]phenanthrene-1,2'-oxolane]-5',7-dione

Details on test material:

- Name of test material (as cited in study report): aldona (ZK 57564)
- Analytical purity: 95.5%
- Lot/batch No.: 59035042
- Expiration date of the lot/batch: Aug 2002

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Duration of test (contact time):

<= 28 d

Initial test substance concentration

Initial conc.:

ca. 8.7 mg/L

Based on:

other: concentration of dissolved oxygen

Results and discussion

% Degradation

Parameter:

% degradation (O2 consumption)

Value:

ca. 30

Sampling time:

11 d

Details on results:

The test compound reached a plateau of degradation on day 11 with 30% degradation, but remained there until the end of the incubation period. The reference material exceeded 60% degradation on day 4. The toxicity control did not indicate an inhibitory effect of aldona

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The reference material exceeded 60% degradation on day 4

Executive summary:

The aerobic biodegradation of aldona was studied in a closed bottle test according to the test guideline OECD 301D. The test compound was incubated at a concentration of 3.2 mg/L over a period of 28 days. Additionally, a reference substance (sodium acetate) and a toxicity control including the test compound and sodium acetate was used. The test compound reached a plateau of degradation on day 11 with 30% degradation, but remained there until the end of the incubation period. The reference material exceeded 60% degradation on day 4, fulfilling the validity criteria. The toxicity control did not indicate an inhibitory effect of aldona. Thus, aldona is not ready biodegradable.