3-oxopregn-4-ene-21,17α-carbolactone

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: well reported guideline GLP study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

3-oxopregn-4-ene-21,17α-carbolactone

EC Number:

213-552-4

EC Name:

3-oxopregn-4-ene-21,17α-carbolactone

Cas Number:

976-70-5

Molecular formula:

C22H30O3

IUPAC Name:

(1R,3aS,3bR,9aR,9bS,11aS)-9a,11a-dimethyl-2,3,3a,3b,4,5,7,8,9,9a,9b,10,11,11a-tetradecahydrospiro[cyclopenta[a]phenanthrene-1,2'-oxolane]-5',7-dione

Details on test material:

- Name of test material (as cited in study report): aldona (57564)
- Analytical purity: 95.5%
- Lot/batch No.: 59035042
- Expiration date of the lot/batch: Aug 2002

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Test organisms

Test organisms (species):

Scenedesmus capricornutum

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 33.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

cell number

Effect concentrations 2

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

ca. 18.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 3

Duration:

72 h

Dose descriptor:

LOEC

Effect conc.:

ca. 33.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Any other information on results incl. tables

In the highest concentration, the growth rate was significantly reduced, although less than 50%. Therefore, the EC50/72h was higher than 33.8 mg/L, the NOEC was 18.8 mg/L, the LOEC 33.8 mg/L.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Aldona is toxic to the green algae Selenastrum capricornutum

Executive summary:

The aquatic toxicity of aldona was studied in a algal growth inhibition test with the green algae Selenastrum capricornutum according to the test guideline OECD 201. The algae were incubated with the test compound over a period of 72 hours in 5 concentrations between 3.2 and 33.8 mg/L, measured by TOC analysis, and a control. In the highest concentration, the growth rate was significantly reduced, although less than 50%. Therefore, the EC50/72h was higher than 33.8 mg/L, the NOEC was 18.8 mg/L, the LOEC 33.8 mg/L.