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EC number: 212-742-4 | CAS number: 865-49-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (non-LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- publication
- Title:
- Sensitization test of chloroform: Comparison of guinea pig maximization test (GPMT) and local lymph node assay (LLNA)
- Author:
- Matsuoka C, Kanazawa Y, Kojima K
- Year:
- 2 002
- Bibliographic source:
- Annual Report of Hatano Research Institute
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.6 (Skin Sensitisation)
- GLP compliance:
- no
- Type of study:
- guinea pig maximisation test
- Justification for non-LLNA method:
- test was conducted in 2002 before testing with LLNA was mandatory
Test material
- Reference substance name:
- Chloroform
- EC Number:
- 200-663-8
- EC Name:
- Chloroform
- Cas Number:
- 67-66-3
- Molecular formula:
- CHCl3
- IUPAC Name:
- chloroform
- Test material form:
- liquid
Constituent 1
In vivo test system
Test animals
- Species:
- guinea pig
- Strain:
- Hartley
- Sex:
- female
- Details on test animals and environmental conditions:
- five weeks old female guinea pigs; temperature between 21 and 25 °C, humidity between 40 and 75 %, air exchange rate 15 times/hour, 12hr light/12 hr darkness
Study design: in vivo (non-LLNA)
Inductionopen allclose all
- Route:
- intradermal
- Vehicle:
- unchanged (no vehicle)
- Concentration / amount:
- no data
Challengeopen allclose all
- Route:
- epicutaneous, occlusive
- Vehicle:
- unchanged (no vehicle)
- Concentration / amount:
- no data
- No. of animals per dose:
- 5 animals for sensitisation test with chloroform, 3 animals for controls
- Details on study design:
- Day 1: intradermal administration of chloroform and Freund's complete adjuvant as primary sensitisation (five test animals)Day 8: open application of 10 % sodium lauryl sulfate as secondary sensitisation (five test animals)Day 9: 48 hours application of occlusive patch as patch sensitisation (five test animals)Day 22: 24 hours application of occlusive patch as challenge (five test animals, three control animals)
- Challenge controls:
- challenge controls with 24 hours application of occlusive patch on Day 22 with three control animals
- Positive control substance(s):
- no
Results and discussion
In vivo (non-LLNA)
Resultsopen allclose all
- Reading:
- 1st reading
- Hours after challenge:
- 48
- Group:
- test chemical
- Dose level:
- no data
- No. with + reactions:
- 5
- Total no. in group:
- 5
- Remarks on result:
- other: Reading: 1st reading. . Hours after challenge: 48.0. Group: test group. Dose level: no data. No with. + reactions: 5.0. Total no. in groups: 5.0.
- Reading:
- 2nd reading
- Hours after challenge:
- 72
- Group:
- test chemical
- Dose level:
- no data
- No. with + reactions:
- 5
- Total no. in group:
- 5
- Remarks on result:
- other: Reading: 2nd reading. . Hours after challenge: 72.0. Group: test group. Dose level: no data. No with. + reactions: 5.0. Total no. in groups: 5.0.
Any other information on results incl. tables
Significant suppression of body weight gain compared to the control group was seen at secondary sensitisation (Day 9) after intradermal chloroform administration (Day 1). Extensive necrosis at the chloroform administration site was observed from the day after administration, and piloerection and decreased spontaneous movement were observed for one week following intradermal administration. In the evaluations at 48 and 72 hours after the start of the challenge, erythema (score 1 or 2, slight to mild) was observed in all eight animals including the three control group animals. This reaction at the challenge site was observed until 8 days after the start of challenge, with a tendency for the erythema to become stronger over time in all 8 animals including the control group, confirming that chloroform is a strong irritant.
Applicant's summary and conclusion
- Interpretation of results:
- not sensitising
- Remarks:
- Migrated information
- Conclusions:
- Sensitisation could not be definitely evaluated due to the strong irritation reaction, but since skin reactions were comparable in the chloroform sensitisation group and the control group, chloroform sensitisation was judged to be negative in GPMT.
- Executive summary:
The skin sensitisation potential of chloroform was tested in the guinea pig maximisation test according to test method B.6 suggested by the European Commission. Sensitisation was tested with 5 female Hartley guinea pigs in the sensitisation group and 3 female animals in the control group.
Significant suppression of body weight gain compared to the control group was seen at secondary sensitisation (Day 9) after intradermal chloroform administration (Day 1). Extensive necrosis at the chloroform administration site was observed from the day after administration, and piloerection and decreased spontaneous movement were observed for one week following intradermal administration. In the evaluations at 48 and 72 hours after the start of the challenge, erythema (score 1 or 2, slight to mild) was observed in all eight animals including the control group. This reaction at the challenge site was observed until 8 days after the start of challenge, with a tendency for the erythema to become stronger over time in all 8 animals including the control group, confirming that chloroform is a strong irritant.
Sensitisation could not be definitely evaluated due to the strong irritation reaction, but since skin reactions were comparable in the chloroform sensitisation group and the control group, chloroform sensitisation was judged to be negative in GPMT.
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