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Diss Factsheets
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EC number: 212-742-4 | CAS number: 865-49-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
Data source
Reference
- Reference Type:
- publication
- Title:
- Toxicity testing of highly volatile chemicals with green algae
- Author:
- Brack W, Rottler H
- Year:
- 1 994
- Bibliographic source:
- Environmental Science and Pollution Research 1(4), 223-228
Materials and methods
- Principles of method if other than guideline:
- A modified cell multiplication inhibition test was applied
- GLP compliance:
- no
Test material
- Reference substance name:
- Chloroform
- EC Number:
- 200-663-8
- EC Name:
- Chloroform
- Cas Number:
- 67-66-3
- Molecular formula:
- CHCl3
- IUPAC Name:
- chloroform
- Test material form:
- liquid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Defined volumes (10 or 100 microlitre) were taken in duplicate from the test cultures with a gas-tight syringe through the septa and injected into 2 mL vials containing 1 mL hexane with trichlorobromomethane as internal standard. Measurement at the beginning and at the end of the assays showed no significant differences and all samples were taken at the end of the assays.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Saturated solutions in growth medium were prepared. Defined aliquots of these solutions were added to the medium. The effective concentrations of the compounds in the alga culture were determined using GC/ECD analysis of extracts at the end of the assays.
Test organisms
- Test organisms (species):
- Chlamydomonas reinhardtii
- Details on test organisms:
- Unicellular freshwater green alga Chlamydomonas reinhardtii (strain number 11-32a SAG) from the University of Göttingen, Germany, were obtained. Precultures and test cultures were grown in the medium for unicellular algae according to Kuhl (1962). Incubation of all cultures were shaken permanently with a frequency of 120 rpm. They were illuminated from above with 130 uE/m2s without light-dark cycle. The photosynthetically effective light was determined with a Quantum Sensor (Licor, Inc.).
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Hardness:
- no data given
- Test temperature:
- 20 +/- 1 °C
- pH:
- 6.5 to 7.5
- Dissolved oxygen:
- no data given
- Salinity:
- no data given
- Nominal and measured concentrations:
- no data given
- Details on test conditions:
- All assays were performed in bipartite vessels. The lower flask contained 50 mL of the CO2 buffer with 2 mol K2CO3/2 mol KHCO3 (35/65), which provided an atmospheric concentration of 1.14 % CO2. The CO2 concentration in the gas phase of the vessel was determined using GC/TCD. 1.2 % were measured without algal growth. Algal growth reduced CO2 concentrations to a minimum of 0.4 %. CO2 deficiency was not expected and not observed. The upper part of the vessel contained 30 mL of the growth medium with the algae. Both liquids were interconnected by their gas phase. The whole system was sealed gas-tight against the atmosphere. The CO2 buffer and the contaminated medium were filled into the test vessel at least 15 hours before the assay was started by adding the algal inoculum. Continuously shaken and temperated to 20 °C, the system was allowed to equilibrate. The inoculum was prepared from 7 day old precultures at a concentration of 3e5 cells/mL. For the test 0.5 mL inoculum was added to 30 mL medium. The cell density in the test cultures amounted to 5e^3 cells/mL at the beginning of the assays.
- Reference substance (positive control):
- yes
- Remarks:
- Cu 2+ (CuSO4)
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 3.61 mg/L
- Nominal / measured:
- meas. (initial)
- Basis for effect:
- growth rate
- Remarks on result:
- other: 2.55-4.72
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 13.3 mg/L
- Nominal / measured:
- meas. (initial)
- Basis for effect:
- growth rate
- Remarks on result:
- other: 11.0-15.77
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The growth of the green alga Chlamydomonas reinhardtii was inhibited by 50 % at a chloroform concentration of 13.3 mg/L.
- Executive summary:
The toxicity of chloroform to the green alga Chlamydomonas reinhardtii was tested in a closed static test during a period of 72 hours at a temperature of 20 +/- 1 °C. The criterion for toxicity was the inhibition of the algal growth rate. The algal growth was inhibited by 10 % at a chloroform concentration of 3.61 (2.55 -4.72) mg/L and by 50 % at a chloroform concentration of 13.3 (11.0 -15.77) mg/L. The EC50 value for algal growth inhibition thus is 13.3 mg chloroform/L.
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