Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1992
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: A reliable and scientifically sound guideline study referring to a read-across substance.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1992
Report Date:
1992

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Qualifier:
according to
Guideline:
EPA OPP 72-2 (Aquatic Invertebrate Acute Toxicity Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material:
PHYSICO-CHEMICAL PROPERTIES
- Molecular weight: 136 g/mol
- Water solubility: 625 g/L at 25°C
- Appearance: white powder

Based on the molecular weights, a concentration of 1200 mg/L sodium trifluoroacetate/L corresponds to 1000 mg trifluoroacetate anion/L.

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
- Concentrations: 0 and 1200 mg/L (nominal) in ISO water
- Sampling: 0h and 48h after test initiation
- Sample storage conditions before analysis: the samples were stored at cool and dark before analysis

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Source: in house culture at Solvay Duphar B.V. since August 1991 and originates from RIVM, The Netherlands
- Age at study initiation: not older than 24 hours
- Feeding during test: no

BREEDING
- Method of breeding: cultured in vessels with 2L reconsituted water (ISO), prepared and aerated at least 24h prior to use
- Feeding during breeding: algal suspension + yeast three times a week
- Culture chamber conditions
- Temperature: 20 ± 1°C
- Illumination: 16 h/d
- Age of Daphnia: not older than 4 weeks

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Test temperature:
19.4 - 20.5°C
pH:
7.7 - 8.0
Dissolved oxygen:
8.4 - 8.6 mg/L
Nominal and measured concentrations:
Nominal: 1200 mg/L and control
Measured: 1220 mg/L (at test start) and 1210 mg/L (at test end)
Details on test conditions:
TEST SYSTEM
- Test vessel: beaker glasses
- Type: open
- Material, size, fill volume: glass, 250 mL (size), 200 mL (fill volume)
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3

TEST MEDIUM / WATER PARAMETERS
- Preparation of dilution water: reconstitued water (ISO)
Deionised water (LRS 1322 830 78115), 294 mg/L CaCl2*2H2O, 123 mg/L MgSO4*7H2O, 64 mg/L NaHCO3, 5.8 mg/L KCl (pH = 7.8; hardness (CaCO3 = ca. 250 mg/L))
- Intervals of water quality measurement: at the beginning (0h) and at the end of the study (48h)

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h/light, 8h/dark

TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 0, 10, 30, 100, 300, 1000 mg/L
- Results used to determine the conditions for the definitive study: yes
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 1 200 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
1 200 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Results with reference substance (positive control):
- Results with reference substance valid? yes
- EC50 (48h): 0.27 mg/L

Applicant's summary and conclusion