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EC number: 247-953-0 | CAS number: 26747-90-0
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
No toxic effects against algae were observed under exposure conditions (72 h-ErL50 > 100 mg/l).
The results are expressed in terms of Effective Loading (EL) (Spoo-Kloeppel, 2015).
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 100 mg/L
- EC10 or NOEC for freshwater algae:
- 100 mg/L
Additional information
"Should read: > 100 mg/L"
The study was conducted in accordance with Commission Regulation (EC) No 761/2009 amending Regulation No 440/2008, Method C.3 ‘Freshwater Alga and Cyanobacteria, Growth inhibition test’ (2009) which is equivalent to OECD Guideline for Testing of Chemicals No. 201 (2006). Exponentially growing algal cells were exposed for a period of 72 hours to a range of concentrations, nominally 4.3, 9.4, 21, 45 and 100 mg/L of Metalink U dissolved in dilution water. Auxiliaries used to prepare the test media were an ultrasonic bath, magnetic stirrer, folded filters and aseptic filters. The cell densities were measured at 24 hour intervals. Inhibition of the algal population was measured as reduction in growth rate (index r), relative to control cultures grown under identical conditions.During the test a temperature range of 21 - 24 °C was maintained in the test vessels. Isocyanatesquickly react with water and different poly urea compounds were formed. Water Accommodated Fractions (WAF) were used to test effects at different effective loadings and no specific analysis was performed. With the sponsor’s agreement, the content of the test item during the exposure period was verified by DOC determination.The pH was measured at the beginning of the test and after 72 hours of exposure. The maintenance of the test item concentration was proved by analytical measurements. In order to avoid an impairment of the test system, an additional replicate was used for analysis and pH measurement at the beginning of the test. Chemical analysis and pH measurement at the end of the test were performed using replicate I of the test concentrations and the control vessels. In order to check whether or not significant amounts of the test item were incorporated into the algal biomass during the test period, a test flask at the limit test concentration without algae was run in parallel to the test concentration. The validity criteria of the test were met. After 72 hours no toxic effects against algae were observed at an effective loading of 100 mg/L. The results are expressed in terms of Effective Loadings (EL).
This toxicity study is classified as acceptable and satisfies the guideline requirements for the Algae study.
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