Reaction product of 3,9-dibromobenzanthrone condensed with 2 equivalents of 1-aminoanthraquinone, subsequently further condensed under oxidative conditions

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Not specified

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1975

Report date:

1975

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

An aerosol of the undiluted test material was generated by passing clean, dry air (-40°C dewpoint) through an Ohio Ball-Jet Nebulizer. The resulting air and aerosol mixture was introduced into the exposure chamber.

GLP compliance:

no

Test type:

traditional method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Reaction product of 3,9-dibromobenzanthrone condensed with 2 equivalents of 1-aminoanthraquinone, subsequently further condensed under oxidative conditions

EC Number:

944-232-9

Molecular formula:

Not available - UVCB substance

IUPAC Name:

Reaction product of 3,9-dibromobenzanthrone condensed with 2 equivalents of 1-aminoanthraquinone, subsequently further condensed under oxidative conditions

Test material form:

liquid

Test animals

Species:

rat

Strain:

other: Charles River

Sex:

male/female

Details on test animals or test system and environmental conditions:

Young adult rats were employed as test animals. The rats were selected after having been under observation for at least 5 days to insure their general health and suitability for testing.The animals were housed in stainless steel cages and permitted a standard laboratory diet plus water ad libitum, except during inhalation exposure.

Administration / exposure

Route of administration:

inhalation: aerosol

Type of inhalation exposure:

whole body

Vehicle:

clean air

Mass median aerodynamic diameter (MMAD):

other: Not specified

Remark on MMAD/GSD:

No data

Details on inhalation exposure:

Test animals were exposed in a specially constructed inhalation chamber. The chamber was designed so that the animals could be introduced into the test atmosphere after the maximum aerosol concentration was established. Each animal was caged separately during exposure to minimize filtration of inspired air by animal fur.An aerosol of the undiluted test material was generated with an Ohio Ball-Jet Nebulizer. The stream of clean, dry air (-40°C dewpoint) was passed through the nebulizer. The resulting air and aerosol mixture was then introduced into the exposure chamber at the top center, dispersed by a baffle plate and exhausted at the bottom of the chamber. Air flow rate through the system was measured with a rotameter connected upstream of the nebulizer. The rotameter was calibrated with a wet-test meter after the exposure was completed. The average nominal aerosol concentration was calculated by dividing the nebulizer weight loss by the total volume of air used during the test.

Analytical verification of test atmosphere concentrations:

no

Duration of exposure:

4 h

Concentrations:

15,750 mg/m3 air

No. of animals per sex per dose:

10 animal (5 male/5 female)

Control animals:

no

Details on study design:

14 day observation period. During the exposure period, observations were made with respect to incidence of mortality and reactions displayed. At the end the exposure period, the rats were returned to their cages for observation.A body weight was determined for each animal prior to inhalation exposure and for each surviving animal at the end of the observation period. The data were recorded as an index to growth.Gross pathologic examinations were scheduled to be conducted upon all animals which might succumb during the test period and upon those sacrificed at the end of the observation period.

Statistics:

Not specified

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

LC50

Effect level:

> 15 750 mg/m³ air (nominal)

Based on:

test mat.

Exp. duration:

4 h

Mortality:

There were no untoward reactions during exposure or the 14-day observation period which followed.

Clinical signs:

other: There were no untoward reactions during exposure or the 14-day observation period which followed.

Body weight:

The average 2-week body weight gains were within the normal limits.

Gross pathology:

Necropsy, performed on all rats at the end of the observation period, did not reveal any gross pathologic alterations.

Other findings:

No further findings reported.

Any other information on results incl. tables

Results

Group No.	Total Number of Animals Male/Female	Nominal Concentration	Mortality Male-Female	Weight Gain Male-Female (grams)
I	5/5	15,750 mg/m^3air	0/5 – 0/5	120-38

 

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

LC50 >15,750 mg/m3 air

Executive summary:

Charles River Rats were exposure for 4 hours.

An aerosol of the undiluted test material was generated by passing clean, dry air (-40°C dewpoint) through an Ohio Ball-Jet Nubulizer. The resulting air and aerosol mixture was introduced into the exposure chamber.

 

There were no untoward reactions during exposure or the 14-day observation period which followed. The average 2-week boy weight gains were within the normal limits. Necropsy, performed on all rats at the end of the observation period, did not reveal any gross pathologic alterations.

 

Acute LC50: >15,750 mg/m³air