PIGMENT ROT 5021 A

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1992-01-23 to 1992-03-27

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP compliant; Guideline Study (OECD 202)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

Part I; 1984

Deviations:

yes

Remarks:

Test concentrations not maintained within the limits given by the guideline at the end of exposure.

GLP compliance:

yes

Test material

Test material information

Constituent 1

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Composite samples (about 150 ml in duplicate) of each test concentration were drawn by mixing identical volumes of the test solutions taken from the approximate center of the test vessels. They were taken immediately before exposure and after 48 hours exposure and kept at - 18°C to - 22°C until analysis.

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 2 g test substance and 18 g from an aqueous solution containing 0.4% TWEEN 80 (polyoxyethylene-sorbitan-monooleate) were mixed during about 24 hours by means of a marble-mills. 2 ml from this solution were mixed with and made up to 2000 ml with water making dilution 1:1000.
- Controls: Blank: water; vehicle: 3.67 mg Tween 80/L
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Tween 80
- Evidence of undissolved material (e.g. precipitate, surface film, etc): A slight to strong deposit was observed at the end of exposure at all test concentrations.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Strain: Straus 1820
- Source: CIBA-GEIGY Ltd., testing facility
- Age at study initiation (mean and range, SD): 0 -24 h
- Method of breeding: Cultures of daphnia are maintained in glass vessels containing approx. 2.5 L of reconstituted water at 20 ± 1 °C (water is renewed partially every second day). At each renewal the daphnia are fed with a suspension of green algae (Scenedesmus subspicatus) supplemented by a suspension of TETRAMIN-extract in such quantities that the food is consumed after 24h.
- Feeding during test: None

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

240 mg CaCO3/L

Test temperature:

21 °C

pH:

7.8

Dissolved oxygen:

after 0 h: 98 - 100%
after 48 h: 96 - 98 %

Nominal and measured concentrations:

nominal: 10, 18, 32, 58, 100 mg/L
measured: 26.4#, 16.0, 31.5, 57.9, 97.6 mg/L (initial)
#this value must be considered as outlier due to problems during sample preparation
measured: 1.4, 1.5, 1.5 1.4 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: beakers with 100 mL test solution
- Type: covered with watch glass
- Material: glass beakers
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4 replicates

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
Reconstituted water in accordance to the guideline. The water was aerated with clean air for at least 24 hours before use.

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: fluorescent light, 16 hours daily
- Light intensity: 1500 lux

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Behavioural abnormalities: none
- Mortality of control: none
- Other adverse effects control: none
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: see details on test solutions

Any other information on results incl. tables

Controls:

Immobilizations in blank : 0 %

Immobilizations in vehicle : 0 %

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

With high probability not acutely harmful to aquatic invertebrates. No toxic effects within the range of solubility.