Registration Dossier

Administrative data

Endpoint:
adsorption / desorption: screening
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2004-11-02 to 2005-01-03
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP compliant; Guideline Study (OECD 121)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2004
Report Date:
2005

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
Deviations:
no
Qualifier:
according to
Guideline:
OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
Deviations:
no
GLP compliance:
yes (incl. certificate)
Type of method:
HPLC estimation method
Media:
soil/sewage sludge

Test material

Reference
Name:
Unnamed
Type:
Constituent
Radiolabelling:
no

Study design

Test temperature:
25 °C

HPLC method

Details on study design: HPLC method:
EQUIPMENT
- Apparatus: Varian autosampler 9100; Varian pump 9010; Varian detector 9050
- Type, material and dimension of analytical (guard) column: LiChrospher 100 CN, part, size 5 µm, 250 mm x 4 mm;
- Detection system: Varian detector 9050;

MOBILE PHASES
- Type: Acetonltrile and water (55:45; v/v); pH 6.8
- pH: pH 6.8
- Solutes for dissolving test and reference substances: acetonitrile;

REFERENCE SUBSTANCES
- Identity: Sodium nitrate; Acetanilide; Isoproturon; Linuron; Pyrazophos; Diclofop-methyl; 2,4-DDT

DETERMINATION OF RETENTION TIMES
- Quantity of test substance introduced in the column: 20 µL;
- Quantity of reference substances:20 mL;


REPETITIONS
- Number of determinations: 3 test runs; 6 reference runs;

EVALUATION
- Calculation of capacity factors k': the capacity factor (k') is calculated from the retention time of the reference items, the test item (tf) and an unretarded item (to): k' = t r - to/to, where tr = Retention time of the test item or reference Item, to = Time a non-sorbed chemical needs to pass the column (dead-time, sodium nitrate solution);
- Determination of the log Koc value: The adsorption coefficient (Koc) is deduced from the capacity factor (k') using a calibration plot of log k' versus log Koc of the selected reference item:
k' = tr - to / to
tr = HPLC retention time of test or reference items [min]
to = HPLC dead time [min] (measurement of the retention time of sodium nitrate)

Results and discussion

Adsorption coefficientopen allclose all
Type:
log Koc
Value:
< 1.25
Temp.:
25 °C
Type:
Koc
Value:
< 18
Temp.:
25 °C

Results: HPLC method

Details on results (HPLC method):
- Retention times of reference substances used for calibration: (6 runs):
Sodium nitrate: 1.15; 1.162 (2 runs);
Acetanilide: average retention time [min]: 2.92;
Isoproturon: average retention time [min]: 3.41;
Linuron: average retention time [min]: 3.86;
Pyrazophos: average retention time [min]: 4.42;
Diclofop-methyl: average retention time [min]: 5.17;
2,4 -DDT: average retention time [min]: 6.53;

- Average retention data for test substance ( 3 runs):
1st Peak: 1.111; 1.122; 1.124;
2nd Peak: 1.385; 1.393; 1.396;
3rd Peak: 1.584; 1.579; 1.572;
4th Peak: 1.712; 1.712; 1.712;

Any other information on results incl. tables

The log of the adsorption coefficient of the test item was determined to be < 1.25. This value indicates that the test item will not be adsorbed by organic carbon in soil. The test item is of very high mobility.

Applicant's summary and conclusion