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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2015-04-09~2015-05-08
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Reference:
Composition 1
Qualifier:
according to
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
GLP compliance:
yes
Test material information:
Composition 1
Inoculum or test system:
activated sludge (adaptation not specified)
Duration of test (contact time):
>= 0 - <= 29 d
Initial conc.:
10 - 20 mg/L
Based on:
test mat.
Parameter:
% degradation (CO2 evolution)
Value:
> 60
Sampling time:
10 d

Table 1 : The amount of CO2 produced in the Ready Biodegradability Test

Time (day)

CO2 produced (mg)

Test article

Blank

Positive control

Toxicity control

Flask1

Flask2

Mean

Flask3

Flask4

Mean

Flask5

Flask6

0

0.33

0.55

0.44

0.33

0.44

0.38

0.33

0.55

3

35.09

34.43

34.76

10.34

9.46

9.90

27.39

26.95

6

12.43

11.55

11.99

7.48

6.60

7.04

25.52

23.65

9

4.62

3.63

4.13

4.18

3.52

3.85

10.89

6.05

12

1.65

1.10

1.38

1.10

1.98

1.54

2.64

2.42

16

3.19

2.20

2.70

1.65

2.64

2.15

2.97

2.75

20

1.21

1.76

1.49

1.43

0.88

1.16

2.42

1.87

24

2.64

3.41

3.03

2.42

1.87

2.15

2.64

2.42

29

1.65

1.43

1.54

1.54

0.77

1.16

1.43

2.42

 

Table 2 : The percent of degradability in the Ready Biodegradability Test

Time (day)

(The accumulated of CO2 produced – The average amount of CO2 produced in control group) / The test substance ( mg )

% ThCO2

Test article

Positive control

Test article

Positive control

0

0.00

0.00

0.12

-0.09

3

0.83

0.58

55.00

27.16

6

1.00

1.20

65.93

55.94

9

1.00

1.43

66.53

66.91

12

1.00

1.47

66.17

68.62

16

1.02

1.50

67.38

69.91

20

1.03

1.54

68.11

71.88

24

1.06

1.55

70.06

72.65

29

1.07

1.56

70.91

73.08

Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
The test groups cultured in the activated sludge for 10 days, the %ThCO2 of the test article was greater than 60%. Therefore, this test article in the
activated sludge belonged to the easy biodegradable substance.
Executive summary:

According to OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test), this test article was readily biodegradable.

Description of key information

This test article should be the ready biodegradable substance. 

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information

The final concentration of the test article and control article in this test was 15 mg/L, and the final concentration of activated sludge was 20 mg/L. The ThCO2 of the test article is 1.51 and that of article of the positive (Sodium benzoate) is 2.14. The result in this test came up to quality requirements, so that the data is valid. The test article in the activated sludge for 10 days, the %ThCO2 of the test article was greater than 60%. According to OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test), this test article was readily biodegradable.