Diethyl ethylmalonate

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2016

Report date:

2016

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Diethyl ethylmalonate

EC Number:

205-093-3

EC Name:

Diethyl ethylmalonate

Cas Number:

133-13-1

Molecular formula:

C9H16O4

IUPAC Name:

1,3-diethyl 2-ethylpropanedioate

Test material form:

liquid

Details on test material:

Test item: diethyl ethylmalonateCAS No.: 133-13-1Physical state: colourless liquidStorage: in cool, dry, well-ventilated place

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, non-adapted

Details on inoculum:

Type of inoculum: aerobic mixed bacterial cultureSource: Municipal wastewater treatment plant, Esztergom-Kertváros

Duration of test (contact time):

28 d

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

PREPARATION OF INOCULUM TEST CULTUREBefore the test 300 ml stock culture was filtered through a filter with 0.2 mm pore size to remove the bigger insoluble particles and then the dry substance content of the filtrate was determined. The measured value was 4.01 g/L. For preparing the inoculum culture 150.01 ml mineral medium was added to 49.96 ml filtered stock culture. The calculated dry mass of inoculum culture was 1 g/L.PREPARATION OF REFERENCE SOLUTIONFor the reference solution 320.02 mg sodium acetate was diluted in 100 ml mineral medium.PREPARATION OF TEST VESSELSFollowing test vessels were prepared and analysed for the study:- Test item (in two replicates)- Blank control (in two replicates)- Reference solution (positive control)- Reference and test item solution (toxicity control)- Sterilized solution of test item (abiotic control)

Reference substance

Reference substance:

acetic acid, sodium salt

Results and discussion

% Degradation

Key result

Parameter:

% degradation (O2 consumption)

Value:

30

Sampling time:

28 d

Remarks on result:

other: mean of two replicates

Details on results:

The biodegradability of the test item on day 14th was 20%.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

The biodegradability of diethyl ethylmalonate was investigated in Ready Biodegradability – Manometric respirometry test (according to OECD 301 F guideline). According to the OECD 301 guideline diethyl ethylmalonate is not ready biodegradable.