Registration Dossier
Registration Dossier
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EC number: 203-254-2 | CAS number: 104-94-9
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- not specified
Test material
- Reference substance name:
- p-anisidine
- EC Number:
- 203-254-2
- EC Name:
- p-anisidine
- Cas Number:
- 104-94-9
- Molecular formula:
- C7H9NO
- IUPAC Name:
- 4-methoxyaniline
- Details on test material:
- - Name of test material (as cited in study report): p-Anisidine
- Molecular formula (if other than submission substance): C7-H9-N-O
- Molecular weight (if other than submission substance): 123.1541g/mol
- Smiles notation (if other than submission substance): c1(ccc(cc1)N)OC
- InChl (if other than submission substance): 1S/C7H9NO/c1-9-7-4-2-6(8)3-5-7/h2-5H,8H2,1H3
- Substance type: Organic
- Physical state: Grey to brown crystalline powder
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Separation method (e.g. HPLC, GC): HPLC
- other: measurement frequency: start of exposure, before renewal of test solution
Test solutions
- Vehicle:
- yes
Test organisms
- Test organisms (species):
- Daphnia magna
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- 20±1 degreeC
- Nominal and measured concentrations:
- 450, 100, 22.2, 4.94, 1.10, 0.244 mg/L (geometric ratio 4.5), control
- Details on test conditions:
- TEST SYSTEM
- Material, size, headspace, fill volume:test liquid volume 100 mL per vessel
- Renewal rate of test solution (frequency/flow rate): replace the total amount of test solution after 24 hours
- No. of organisms per vessel: 20 per concentration (5 per vessel)
- No. of vessels per concentration (replicates): 4
OTHER TEST CONDITIONS
- Photoperiod: 16 hours light - 8 hours dark
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 4.12 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks:
- Immobilization
- Remarks on result:
- other: 95 % CL 2.76 - 6.13 mg/L
Applicant's summary and conclusion
- Conclusions:
- EC50 for p-Anisidine in Daphnia magna was found to be 4.12 mg/l, when exposed for 48h under semi-static condition.
- Executive summary:
In the study Short-term toxicity to aquatic invertebrates for p-Anisidine, Daphnia magna was exposed at nominal and measured concentrations of 450, 100, 22.2, 4.94, 1.10, 0.244 mg/L for duration of 48 hr. Immobilization was observed at concentration of 4.12 mg/l. Therefore EC50 for p-Anisidine
was considered to be 4.12 mg/l in Daphnia magna when exposed for 48h under semi-static condition.
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