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EC number: 222-656-9 | CAS number: 3567-66-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Experimental test result performed using standard OECD test guidelines
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 016
- Report date:
- 2016
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Principles of method if other than guideline:
- This study was designed to assess the toxic effects of the test compound on the Zebra Fish (Danio rerio).Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203.
- GLP compliance:
- yes
Test material
- Reference substance name:
- Disodium 5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonate
- EC Number:
- 222-656-9
- EC Name:
- Disodium 5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonate
- Cas Number:
- 3567-66-6
- Molecular formula:
- C16H13N3O7S2.2Na
- IUPAC Name:
- disodium 5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonate
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- - Name of test material (as cited in study report): disodium 5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonate- Molecular formula (if other than submission substance): C16-H13-N3-O7-S2.2Na- Molecular weight (if other than submission substance): 467.3889 g/mol- Substance type: Organic - Physical state: Reddish brown powder- Analytical purity: 93.54% (Pure dye content)- Lot/batch No.: FG/15-16/2641- Expiration date of the lot/batch: December, 2023-Storage Conditions-•Ambient Temp (23 to 27 °C)
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM- Common name:Zebra Fish - Strain:Danio rerio - Source:Unique Aqua Fish Corner- Age at study initiation (mean and range, SD): No data available.- Length at study initiation (length definition, mean, range and SD):3.23 cm - Weight at study initiation (mean and range, SD):0.203 g (approx.)0.322 g- Feeding during test: No - Food type:Standard brand feed,- Frequency: once dailyACCLIMATION- Acclimation period: 6 days - Acclimation conditions (same as test or not):yes - Type and amount of food:Standard brand feed,- Feeding frequency:once daily up to sixth day of acclimatization- Health during acclimation (any mortality observed): No
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- 147.5
- Test temperature:
- 25°C
- pH:
- 7.01
- Dissolved oxygen:
- 7.06 mg/L
- Salinity:
- No data available.
- Nominal and measured concentrations:
- 100 mg/L.
- Details on test conditions:
- TEST SYSTEM- Test vessel: The test vessels used were all plastic aquaria having the water capacity of 5liters.- Type (delete if not applicable): open / closed- Material, size, headspace, fill volume:- Aeration:Aeration in test vessels was provided 1 day before the start of experiment- Type of flow-through (e.g. peristaltic or proportional diluter):- Renewal rate of test solution (frequency/flow rate):- No. of organisms per vessel: 4- No. of vessels per concentration (replicates):2- No. of vessels per control (replicates):1TEST MEDIUM / WATER PARAMETERS- Source/preparation of dilution water: The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.OTHER TEST CONDITIONS- Adjustment of pH:- Photoperiod: 12 hours light and 12 hours darkness- Light intensity:
- Reference substance (positive control):
- not specified
Results and discussion
Effect concentrations
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
Any other information on results incl. tables
- Sublethal observations / clinical signs:
Mortality and symptoms
NominalConcentration
(mg/l)
Number of fish
Mortality (cumulated) after
3 h
6 h
24h
48h
72h
96h
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
0 (control)
4
4
-
-
-
-
-
-
-
-
-
-
-
-
100
4
4
-
-
-
-
-
-
-
-
-
-
-
-
Visible Symptoms:
NominalConcentration
(mg/l)
Number of fish
Symptoms after
3 h
6 h
24h
48h
72h
96h
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
0 (control)
4
4
-
-
-
-
-
-
-
-
-
-
-
-
100
4
4
-
-
-
-
-
-
-
-
-
-
-
-
Explanation of symptoms:
A:apathy
N:Narcotic-like state
C:Swimming near water surface
M: Slow movements compared to control
NS:No abnormal symptoms
S:Surfacing behaviour
L:Loss of equilibrium
SM:Shimming movement
H1:Hyperactivity
H2:Hypo activity
B:Bending behavior
F:Fin rot
IS:Invert Swimming
SB:Swimming behaviour
RF:Respiratory Function
P:Pigmentation
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for CAS No. 3567-66-6 on Zebra Fish were found to be>100mg/L.
- Executive summary:
Fish Acute Toxicity test according to OECD Guideline 203 was conducted for __________. The nominal concentration selected for the experiment were ____________ and Zebra fish were exposed to these concentration for 96 hours. The lethal concentrations LC50 was found to be >100 mg/L.
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