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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Eye irritation

Currently viewing:

Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Data is from opinions on health and safety risks (chemical, biological, mechanical and other physical risks)

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
2007

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: refer below principle
Principles of method if other than guideline:
Study conduct to determine the irritation effects of test material Acid Red 33 on rabbit eye and rritation was scored according to Draize.
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Disodium 5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonate
EC Number:
222-656-9
EC Name:
Disodium 5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonate
Cas Number:
3567-66-6
Molecular formula:
C16H13N3O7S2.2Na
IUPAC Name:
disodium 5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonate
Test material form:
solid: particulate/powder
Remarks:
migrated information: powder
Details on test material:
- Name of test material (as cited in study report): disodium 5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonate- Molecular formula (if other than submission substance): C16H13N3O7S2.2Na - Molecular weight (if other than submission substance): 467.389 - Smiles notation (if other than submission substance): [Na+].[Na+].Nc1cc(S(=O)(=O)[O])cc2cc(S(=O)(=O)[O-])c(N=Nc3ccccc3)c(O)c12- Substance type: Organic - Physical state: Solid

Test animals / tissue source

Species:
rabbit
Strain:
not specified
Details on test animals or tissues and environmental conditions:
No data

Test system

Vehicle:
water
Controls:
yes
Amount / concentration applied:
0.2 ml of a 10% aqueous solution (20 mg)
Duration of treatment / exposure:
test material was applied twice daily, five times weekly
Observation period (in vivo):
four weeks
Number of animals or in vitro replicates:
six or more albino rabbits
Details on study design:
SCORING SYSTEM: Draize

Results and discussion

In vivo

Results
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
other: 20 days
Score:
0
Max. score:
6
Reversibility:
not specified
Remarks on result:
other: not irritating to the rabbit eye

Any other information on results incl. tables

 

Before 1 application on day:

3 days after last application

1st hour after 1st application on day 5

Irritation score

2

5

10

20

0

2

0

0

0

0

Applicant's summary and conclusion

Interpretation of results:
not irritating
Remarks:
Migrated informationCriteria used for interpretation of results: EU
Conclusions:
The study concluded that the test substance was not irritating to the rabbit eye.
Executive summary:

0.2 ml of a 10% aqueous solution (20 mg) or suspension of the test material was applied twice daily, five times weekly, for four weeks to the conjunctival sac of one eye of each of a group of six or more albino rabbits (40 applications). One hour after each application, the eyes were examined for evidence of staining and the irritation was scored according to Draize.

The study concluded that the test substance was not irritating to the rabbit eye.