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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: OECD Guideline Study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1991
Report date:
1991

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes

Test material

Constituent 1
Reference substance name:
Ethane, 2-Chloro-1,1-Dimethoxy-
IUPAC Name:
Ethane, 2-Chloro-1,1-Dimethoxy-
Constituent 2
Reference substance name:
97-97-02
IUPAC Name:
97-97-02
Constituent 3
Chemical structure
Reference substance name:
2-chloro-1,1-dimethoxyethane
EC Number:
202-624-0
EC Name:
2-chloro-1,1-dimethoxyethane
Cas Number:
97-97-2
Molecular formula:
C4H9ClO2
IUPAC Name:
2-chloro-1,1-dimethoxyethane
Test material form:
other: liquid
Details on test material:
- Name of test material (as cited in study report): Chloroacetaldehyde dimethylacetal
- Physical state: colourless liquid
- Solubility in water: 52 g/L at 20°C
- pH: 3.4 at 10g/L (20°C)

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: zebra danio
- Source: West Aquarium, D-Bad Lauterberg
- Length at study initiation: 2-3cm
- Weight at study initiation: 0.35g in average
- Feeding during test: no

ACCLIMATION
- Acclimation period: 7d in test water befor study initiation
- Acclimation conditions (same as test or not): yes
- Type and amount of food: flakes (Tetra Min Hauptfutter)
- Feeding frequency: 3 times/week, last feeding 24h before study initiation
- Health during acclimation (any mortality observed):

QUARANTINE
- Duration: 12d in quarantine
- Health/mortality: no external visible illnesses/0.25%

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96
Remarks on exposure duration:
h

Test conditions

Hardness:
2.83 - 3.14 mmol/L
Test temperature:
21 +/- 1°C
pH:
7.39-7.49
Nominal and measured concentrations:
nominal: 0, 25, 50, 100, 200, 400 mg/L
measured: 0, 26.31/24.76, 47.78/48.84, 99.46/92.11, 205.48/204.58, 405.81/396.37 mg/L
Details on test conditions:
TEST SYSTEM
- Material, size, headspace, fill volume: 10L
- Aeration: no
- Renewal rate of test solution (frequency/flow rate): every 24h
- No. of organisms per vessel: 10

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tab water
Dilution water:
CaCl2: 2 mmol/L, MgSO4: 0.5 mmol/L, NaHCO3: 0.75 mmol/L, KCl: 0.075 mmol/L
The sum of the Calcium- and Magnesium ions in the dilution water is 2.5 mmol/L. The relation of Ca : Mg ions 4 : 1 and Na : K ions 10 : 1. The acid capacity of the solution is 0.8 mmol/L. The water will be aerated until oxygen saturation; afterwards the stock solutions will be mixed with 500mL dilution water.

Stock solutions:
(1): 0.5 g/L Chloroacetaldehyde dimethylacetal, (2): 1 g/L Chloroacetaldehyde dimethylacetal, (3): 2 g/L Chloroacetaldehyde dimethylacetal, (4) 4 g/L Chloroacetaldehyde dimethylacetal, (5): 8 g/L Chloroacetaldehyde dimethylacetal.
stock solution : dilution water is 1 : 20

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 light/8 dark
- Light intensity: 2000 lux
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
50 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
50 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
200 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Details on results:
- Behavioural abnormalities: balance disorders in 1 fish (24h) and 2 fish (48h)
- Observations on body length and weight: normal: 2,8 cm in average and 0.35g in average
- Mortality of control: no
- Other adverse effects control: no

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Chloroacetaldehyde dimethylacetal was tested according to OECD Guideline 203 tested in zebra danio (Brachydanio rerio (new name: Danio rerio)). 2 out of 6 died, so the mortality was 1/3 at a concentration of 100 mg/l.
Executive summary:

Chloroacetaldehyde dimethylacetal was tested according to OECD Guideline 203 tested in zebra danio (Brachydanio rerio (new name: Danio rerio)). The LD50 is > 100 mg/l.