Registration Dossier
Registration Dossier
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EC number: 202-869-3 | CAS number: 100-60-7
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
The 16-h EC50 and 16-h EC10 values are 176.5 and 108.3 mg/L (nominal), respectively. The inhibition of the degradation activity of activated sludge is not anticipated when introduced to biological treatment plants in appropriate low concentrations.
Key value for chemical safety assessment
Additional information
The toxicity of the substance to microorganisms was investigated in a bacterial growth inhibition study according to DIN 38412, part 8 (BASF, 1987). In this study cultures of bacteria (Pseudomonas putida) were exposed to nominal test substance concentrations of 0 (control), 7.8, 15.6, 31.3, 62.5, 125, 250, 500, 1000 and 2000 mg/L for 16 hours. Test concentrations were not analytically verified. In the study a significant increase in pH was noted which was most pronounced at test concentrations of 125 mg/L and higher. Bacterial growth (inhibition) was determined optically by means of spectrophotometry at a wavelength of 436 nm and compared to a blank control. Up to a test concentration of 62.5 mg/L a slight increase in cell growth could be observed. At a test concentration of 125 mg/L 15.4% cell growth inhibition was recorded. At higher test concentrations growth inhibition neared 100%. Based on these findings, the 16 -h EC50 and 16 -h EC10 values were determined at 176.5 mg/L and 108.3 mg/L, respectively.
From the bacterial growth inhibition study no data from pH-adjusted test concentrations are available. As deviating pH values are known to be able to affect microbial growth significantly, the increased pH values in the test may have contributed, at least in part, to the effects observed. In the absence of data from pH-adjusted data, the effect values observed may be considered to be worst case.
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