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Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vitro / ex vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Well documented study according to GLP and internationally accepted standard procedure (comparable to guideline)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2006
Report date:
2006

Materials and methods

Test guideline
Qualifier:
no guideline available
Principles of method if other than guideline:
The assessment of the eye irritation potential is included in international regulatory requirements for the testing of chemicals. The HET-CAM Test is an alternative in vitro method for testing of severe eye/mucous membrane damage using the chorionallantoic membrane of fertilized, incubated hen eggs.
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
N-(n-octyl)-2-pyrrolidinone
EC Number:
403-700-8
EC Name:
N-(n-octyl)-2-pyrrolidinone
Cas Number:
2687-94-7
Molecular formula:
C12 H23 N O
IUPAC Name:
1-octylpyrrolidin-2-one
Details on test material:
- Name of test material (as cited in study report): N-(N-Octyl)pyrrolidone; Substance No. 91/453
- Physical state: colorless liquid
- Analytical purity: 99.3 %
- Lot/batch No.: FS 20628
- Storage condition of test material: refrigerator

Test animals / tissue source

Species:
other: Hen eggs in vitro

Results and discussion

In vitro

Results
Irritation parameter:
other: threshold concentration of test substance for effects indicating serious eye damage (mean time to coagulation < 90 sec)
Remarks on result:
positive indication of irritation
Remarks:
threshold concentration of NOP: 17 %

Any other information on results incl. tables

 

Concentration

Egg-No.

Time (sec) until appearance of :

Grading effects

Additional findings

Haemorrhagia

Coagulation

Haemorrhagia

Coagulation

Test substance 10 % in Pluronic PR6200

1

135

150

2

2

iv

2

128

133

2

2

iv

3

159

200

2

1

iv

Mean

n = 3

141

161

2

2

 

 

Concentration

Egg-No.

Time (sec) until appearance of :

Grading effects

Additional findings

Haemorrhagia

Coagulation

Haemorrhagia

Coagulation

Test substance 20 % in Pluronic PR6200

1

60

76

1

2

iv

2

44

56

2

2

iv

Mean

n = 1

52

66

2

2

 

 

Concentration

Egg-No.

Time (sec) until appearance of :

Grading effects

Additional findings

Haemorrhagia

Coagulation

Haemorrhagia

Coagulation

Test substance 40 % in Pluronic PR6200

1

36

46

2

2

iv

Mean

n = 1

36

46

2

2

 

 

Applicant's summary and conclusion

Interpretation of results:
corrosive

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