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EC number: 440-930-8 | CAS number: 330198-48-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- October 8, 2001 till October 10, 2001
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study according to OECD guideline 209 (Activated Sludge, Respiration Inhibition Test) under GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 002
- Report date:
- 2002
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- -
- EC Number:
- 440-930-8
- EC Name:
- -
- Cas Number:
- 330198-48-6
- Molecular formula:
- C19H28N2O3 / C21H32N2O3 / C23H36N2O3 / C29H50N2O4 / C31H54N2O4 / C33H58N2O4 / C35H62N2O4 / C37H66N2O4
- IUPAC Name:
- Condensation products of 4-methyl-m-phenylene diisocyanate with alcohols, C10-14 (even numbered)
- Details on test material:
- Identity: ZP-TIX 1014
Batch No.: NW-01-028
Purity: >99%
Appearance: white solid
Storage: At room temperature at about 20°C (in the dark)
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The nominal final test item concentrations tested were 10, 32, 100, 320 and 1000 mg/L. Concentrations exceeding 1000 mg test item per liter were not tested.
According to the results of a pre-test, no stock solution could be prepared due to the low water solubility of ZP-TIX 1014.Therefore, test item amounts of 5.1, 16.2, 50.3, 162 and 501 mg were directly weighed (by means of an analytical balance) into the test flasks. Then 284 mL of tap water were added to each flask. The test item was mixed into the tap water by ultrasonic treatment over 15 minutes and intense stirring over 24 hours at room temperature in the dark. Then, 16 mL of synthetic wastewater and 200 mL of the activated sludge inoculum were added .
The test item is a poorly soluble mixture of isocyanates and it is generally known, that these compounds degrade fast in water. Therefore, the degradation products were tested in addition to the undissolved fraction of the test item dispersion. A long stirring period of 24 hours was chosen, to enrich the test water with the maximum concentration of degradation products within a practically acceptable time period.
In addition to the test media, two controls (tap water, synthetic wastewater and activated sludge inoculum, without test item) and the reference item 3,5-dichlorophenol (positive control) at nominal concentrations of 5, 16 and 50 mg/L were tested in parallel under
otherwise identical test conditions.
The test was performed in 1000-mL glass flasks. The test flasks were labelled with the necessary information to assure unmistakable identification.
At the start of the test 200 mL activated sludge inoculum (see Section 2.3) with a sludge concentration of 2.5 9 dry weight/L (corresponding to about 1.0 9 dry material per liter test medium) was added. The sludge was added in time intervals of 15 minutes (an arbitrary but convenient interval) first to a control, then to the test solutions of the reference item, then to the test solutions of the test item, and finally to the second control.
During the incubation period of exactly 3 hours all test media and the controls were continuously aerated with compressed air at a flow of approximately one liter per minute. The concentration of dissolved oxygen did not drop below 2.5 mg/L during the incubation period, and just before the measurements of the respiration rates the dissolved oxygen concentrations were at least 6.6 mg/L. The temperature in the test media, measured in one control, was 21°C at the start and 20°C at the end of the incubation period.
For test media containing the reference item a stock solution of 3,5-dichlorophenol was prepared according to the test guidelines: 0.5 9 of 3,5-dichlorophenol was dissolved in 10 mL 1 M NaOH and diluted to about 30 mL with purified water. Excess of NaOH was neutralized with 0.5 mol/L H2S04 to the point of incipient precipitation. Thereafter, the mixture was made up to one liter with purified water. The pH was determined to be 7.8 and the final concentration amounted to 500 mg/L. Aliquots of this 3,5-dichlorophenol stock solution were mixed with synthetic wastewater and tap water in the respective test flasks to obtain the desired concentrations.
The pre-test to the solubility of the test item was not performed in compliance with the GLP. Regulations and therefore are excluded from the Statement of Compliance. However, the raw data, respectively copies of raw data will be archived under the RCC study number of the present study.
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- The sludge was washed once with tap water by centrifugation and the supernatant liquid phase was decanted. A homogenized aliqout of the final sludge supsension was weighed, thereafter dried and the ration of wet to dry weight was calculated.
Based on this ratio, an aliquot of washed sludge was suspended in tap water to obtain a cconcentrations equivalent to 3 g dry material per liter.
During the holding of two and a half days prior to use, the sludge was fed with 50 ml synthetic wastewater /liter / day and was kept at room temperature under continuous aeration until use. Immediately before use, the dry weight of the activated sludge was measured again in the inoculum used in the test. The pH of the activated sludge inoculum was adjusted from 8.4 to 7.6 with a diluted sulfuric acid solution.
Synthetic wastewater:
16 g peptone
11 g meat extract
3 g urea
0.7 g NaCI
0.4 g CaCI2 * 2H20
0.2 g MgS04 * 7H20
2.8 g K2HP04
was filled up to a final volume of 1 liter with deionized water.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 3 h
Test conditions
- Test temperature:
- The temperature in the test media, measured in one control, was 21 °C at the start and 20 °C at the end of the incubation period.
- pH:
- The pH of the activated sludge inoculum was adjusted from 8.4 to 7.6 with a diluted sulfuric acid solution
- Dissolved oxygen:
- The concentration of dissolved oxygen did not drop 2.5 mg/L during the incubation period, and just before the measurements of the respiration rates the dissolved oxygen concentrations were at leat 6.6 mg/L.
- Salinity:
- Synthetic wastewater:
16 g peptone, 11 g meat extract, 3 g urea, 0.7 g NaCl, 0.4 g CaCl2.2H2O, 0.2 g MgSO4.7H2O, 2.8 g K2HPO4 - Nominal and measured concentrations:
- The nominal concentrations tested were 10, 32, 100, 320 and 1000 mg/l.
Concentrations exceeding 1000 mg/l were not tested.
Down to the lowest test concentration of 10 mg/l a part of the test item was suspended in the test media - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 1 000 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks:
- < 15 %
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Conc. based on:
- test mat.
- Details on results:
- Up to and including the concentration of 1000 mg/l the test item and its degradation products, respectively, had no significant inhibitory effect (<15%) on the respiration rate of activated sludge after the incubation period of three hours.
The 3-hour NOEC (EC15) of the substance to activated sludge microorganisms was at least 1000 mg/l or higher. The 3-hour EC20, EC50 and EC80 could not be calculated, but were clearly higher than 1000 mg/l. - Results with reference substance (positive control):
- The 3-hour EC50 of the reference item 3,5-dichlorophenol (positive control) was calculated to be 16 mg/L (the 95 % confidence limits were not calculable). The 3-hour EC50 is witin the guideline-recommended range of 5 - 30 mg/L, confirming the suitability of the activated sludge used.
Any other information on results incl. tables
Table: Influence of ZP-TIX 1014 on oxygen consumption of activated sludge | ||||||||
Flask | Test chemical | Nominal | Oxygen | Inhibition | pH values | Oxygen | ||
concentration | consumption | concentration | ||||||
of test | rate | (mg O) | ||||||
chemical | ||||||||
(mg/I) | (mg 02/l min-1) | (%) | start | end | start | end | ||
1 | Control | 0 | 0.968 | 8.0 | 8.7 | 6.8 | 6.9 | |
10 | Control | 0 | 1.098 | 8.0 | 8.7 | 7.9 | 7.5 | |
Mean | 1.033 | |||||||
Deviation (%) | 13.4 | |||||||
5 | ZP-TIX 1014 | 10 | 1.002 | 3.0 | 7.9 | 8.7 | 7.9 | 7.2 |
6 | ZP-TIX 1014 | 32 | 0.988 | 4.4 | 8.0 | 8.7 | 8.0 | 7.9 |
7 | ZP-TIX 1014 | 100 | 1.026 | 0.7 | 8.0 | 8.7 | 7.2 | 8.0 |
8 | ZP-TIX 1014 | 320 | 1.063 | -2.9 | 8.0 | 8.7 | 7.9 | 7.6 |
9 | ZP-TIX 1014 | 1000 | 1.155 | -11.8 | 8.0 | 8.7 | 7.9 | 6.6 |
Table: Influence of 3,5-dichlorophenol on oxygen consumption of activated sludge | ||||||||
Flask | Test chemical | Nominal | Oxygen | Inhibition | pH values | Oxygen | ||
concentration | consumption | concentration | ||||||
of test | rate | (mg O) | ||||||
chemical | ||||||||
(mg/I) | (mg 02/l min-1) | (%) | start | end | start | end | ||
1 | Control | 0 | 0.968 | 8.0 | 8.7 | 6.8 | 6.9 | |
10 | Control | 0 | 1.098 | 8.0 | 8.7 | 7.9 | 7.5 | |
Mean | 1.033 | |||||||
Deviation (%) | 13.4 | |||||||
2 | 3,5-dichlorophenol | 5 | 0.812 | 21.4 | 7.7 | 8.5 | 7.0 | 7.7 |
3 | 3,5-dichiorophenol | 16 | 0.590 | 42.9 | 7.8 | 8.1 | 7.5 | 7.5 |
4 | 3.5-dichlorophenol | 50 | 0.179 | 82.7 | 7.8 | 8.4 | 7.8 | 8.3 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Up to and including the concentration of 1000 mg/l the test item and its degradation products, respectively, had no significant inhibitory effect (< 15 %) on the respiration rate of activated sludge after the incubation period of three hours. The 3-hour NOEC (EC15) of the substance to activated sludge microorganisms was at least 1000 mg/l or higher.
- Executive summary:
The inhibitory effect of the test item on the registration rate of aerobic wastewater microorganisms of activated sludge was investigated in a 3 -hour respiration inhibition test according to the EU Commission Directive 87/302/EEC, and the OECD Guideline for testing of chemicals, No. 209.
The nominal concentrations tested were 10, 32, 100, 320 and 1000 mg/l. Concentrations exceeding 1000 mg/l were not tested. Down to the lowest test concentration of 10 mg/l a part of the test item was suspended in the test media.
Two controls (tap water, synthetic wastewater and activated sludge inoculum, without test item) and three different concentrations of the reference item 3,5 -dichlorophenol (5, 16 and 50 mg/l) were tested in parallel. The results of these treatments confirmed the suitability of the activated sludge and the method used.
Up to and including the concentration of 1000 mg/l the test item and its degradation products, respectively, had no significant inhibitory effect (< 15 %) on the respiration rate of activated sludge after the incubation period of three hours. The 3-hour NOEC (EC15) of the substance to activated sludge microorganisms was at least 1000 mg/l or higher. The 3-hour EC20, EC50 and EC80 could not be calculated, but were clearly higher than 1000 mg/l.
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