Registration Dossier

Administrative data

Key value for chemical safety assessment

Additional information

The substance 1-Ethyl-3-methyl-1-imidazolium chloride (EMIM CI) was tested for its mutagenic potential based on the ability to induce point mutations in selected loci of several bacterial strains, i.e. Salmonella typhimurium and Escherichia coli, in a reverse mutation assay. The dose range was 20 µg - 5000 µg/plate for SPT and PIT with and without metabolic activation. No precipitation of the test substance was found. A weak bacteriotoxic effect was occasionally observed using strain TA 1537 depending on the test conditions at 5 000 μg/plate. An increase in the number of his+ or trp+ revertants was not observed in the standard plate test or in the preincubation test either without S9 mix or after the addition of a metabolizing system. According to the results of the present study, the test substance 1-Ethyl-3-methyl-1- imidazolium chloride (EMIM CI) is not mutagenic in the Salmonella typhimurium / Escherichia coli reverse mutation assay under the experimental conditions chosen here. (BASF, 2007)

There is also an Bacterial Mutagenicity study from NTP available (only abstract). 1-ethyl-3-methylimidazolium chloride was tested in bacterial strains TA 100, TA 98 and eColi pKM101 in concentrations up to 10000 µg/plate. In this study the test substance was negative. (NTP, 2014)


Justification for selection of genetic toxicity endpoint
Only available study

Short description of key information:
Ames: negative

Endpoint Conclusion: No adverse effect observed (negative)

Justification for classification or non-classification

Based on the results, the test item is no subject to classification and labelling according to Regulation (EC) No 1272/2008 (CLP).