Ricinoleyl monomaleate triglyceride

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

12 November 1999 - 21 February 2000

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP study according to international guidelines but lacking analytical confirmation of actual test concentrations.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2000

Report date:

2000

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method C.3 (Algal Inhibition test)

Test guideline 2

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

yes

Test material

Reference

Name:

Unnamed

Type:

Constituent

Test material form:

liquid: viscous

Details on test material:

Batch: T4649
Expiry date: 22 April 2001
Storage at 10 to 30 dC

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

The analytical method was suitable only for measurement of actual concentrations in samples taken from the stock solutions in acetone. Samples were also taken from the test media at the start and the end of the test. Analysis of the test concentration samples was attempted but resulted in most cases in non-dectable values. Therefore, final evaluation of the results was based on nominal concentrations.

Test solutions

Vehicle:

yes

Details on test solutions:

Identity and concentration of auxiliary solvent for dispersal: acetone 0.1 ml/l. Stock solutions in acetone contained 60 or 120 g/L. These were used to prepare the final test solutions.

Test organisms

Test organisms (species):

Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Test conditions

Test temperature:

23.8 - 25.9 dC

pH:

7.5 - 9.6

Nominal and measured concentrations:

Nominal: 6 mg/L - Stock: 60 g/L - Actual: 54 g/L
Nominal: 12 mg/L - Stock: 120 g/L - Actual: 108 g/L

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC0

Effect conc.:

ca. 12 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 12 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The test substance has proven to be non-toxic to fresh-water algae at levels at and above its solubility in water.