1-chloro-2,3-epoxypropane

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

not specified

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: No data on GLP but was a guideline study.

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))

Deviations:

no

Remarks:

Not specified in report

GLP compliance:

not specified

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
no data

Oxygen conditions:

aerobic

Inoculum or test system:

mixture of sewage, soil and natural water

Details on inoculum:

Sludge was obtained from 10 locations (Fukogawa city sewage, Fukashiba industry sewage plant, Nakahama city sewage plant, Ochiai city sewage plant, Kitakami river, Shinano river, Yoshino river, Lake Biwa, Hiroshima bay and Dookai bay) four times each year. Inoculum is cultured in the laboratory on a glucose/peptone medium for 30-120 days prior to use in the test.

Duration of test (contact time):

14 d

Initial conc.:

100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

Four test solutions were prepared: 1) water + test substance, 2) sludge + test substance, 3) sludge + aniline and 4) control blank.

Preliminary study:

no data

Test performance:

no data

Parameter:

% degradation (O2 consumption)

Value:

18

Sampling time:

14 d

Details on results:

no additional information available

Results with reference substance:

no additional information available

Epichlorohydrin was not readily biodegradable in this test.

Interpretation of results:

other: not readily biodegradable

Conclusions:

The biodegradability of epichlorohydrin was examined using the MITI test. Epichlorohydrin was not readily biodegradable in this test at a concentration (100 mg/l) where inhibition of the inoculum cannot be ruled out. No information on inhibitory effects of the test material on the inoculum is available.

Executive summary:

The biodegradability of epichlorohydrin was examined using the MITI test. Epichlorohydrin was not readily biodegradable in this test.

Reference 2

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: No data on GLP but was a guideline study.

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))

Deviations:

not specified

GLP compliance:

not specified

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
no data

Oxygen conditions:

aerobic

Inoculum or test system:

mixture of sewage, soil and natural water

Details on inoculum:

Sludge was obtained from 10 locations (Fukogawa city sewage, Fukashiba industry sewage plant, Nakahama city sewage plant, Ochiai city sewage plant, Kitakami river, Shinano river, Yoshino river, Lake Biwa, Hiroshima bay and Dookai bay) four times each year. Inoculum is cultured in the laboratory on a glucose/peptone medium for 30-120 days prior to use in the test.

Duration of test (contact time):

14 d

Initial conc.:

100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

Four test solutions were prepared: 1) water + test substance, 2) sludge + test substance, 3) sludge + aniline and 4) control blank.

Preliminary study:

no data

Parameter:

% degradation (O2 consumption)

Value:

68

Sampling time:

14 d

Parameter:

% degradation (TOC removal)

Value:

84.3

Sampling time:

14 d

Remarks on result:

other: Test terminated after 14 d.

Parameter:

% degradation (test mat. analysis)

Value:

92.5

Sampling time:

14 d

Remarks on result:

other: Test terminated after 14 d.

Details on results:

no additional information provided

Results with reference substance:

Result for references substance not given in the MITI database. For the study results to be accepted, the percentage biodegradation of aniline has to be >40 and >60% in 7 and 14 days, respectively.

The test material, 3 -chloro-1,2 -propylene glycol, meets the definition of readily biodegradable in the OECD 301C test.

Interpretation of results:

readily biodegradable

Conclusions:

The test material, 3 -chloro-1,2 -propylene glycol, meets the definition of readily biodegradable in the OECD 301C test.

Executive summary:

The test material, 3 -chloro-1,2 -propylene glycol, which is the initial degradation product of epichlorohydrin, meets the definition of readily biodegradable in the OECD 301C test.

Reference 3

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

not specified

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The study was not conducted in accordance to any guidelines and is a non GLP study.

Principles of method if other than guideline:

Ready biodegradability study using non-acclimated organisms over an observation period of 20 days and comparison of BOD and ThOD as parameter.

GLP compliance:

not specified

Oxygen conditions:

aerobic

Remarks:

no additional information specified

Inoculum or test system:

activated sludge, non-adapted

Details on inoculum:

no data

Duration of test (contact time):

20 d

Parameter:

% degradation (O2 consumption)

Value:

48

Sampling time:

20 d

Remarks on result:

other: After 5, 10 and 15 days, 4, 33 and 36%, respectively, of the theoretical oxygen consumption was used.

Details on results:

Theoretical oxygen consumption was 1.21

Using unadapted mirco-organisms, 48% of the epichlorohydrin was biodegraded in 20 days.

Conclusions:

Using non-adapted organisms, 48% of the epichlorohydrin was biodegraded in 20 days based on BOD/ThOD.

Executive summary:

Using non-adapted organisms, 48% of the epichlorohydrin was biodegraded in 20 days based on BOD/ThOD.

Description of key information

The hydrolysis product of Epichlorohydrin is readily biodegradable. The Japanese Institute of Technology and Evaluation concludes Epichlorohydrin to be readily biodegradable based on this result.

Key value for chemical safety assessment

Biodegradation in water:

readily biodegradable

Type of water:

freshwater

Additional information

Biodegradability of Epichlorohydrin has been investigated following the OECD 301 C (MITI) protocol at 100 mg/l; a concentration that may be inhibitory to the inoculum (no information on inhibition available). However, the hydrolysis product of Epichlorohydrin is readily biodegradable. The Japanese Institute of Technology and Evaluation concludes Epichlorohydrin to be readily biodegradable based on this result.