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Biodegradation in water: screening tests

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Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
not specified
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: No data on GLP but was a guideline study.
Qualifier:
according to
Guideline:
OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
Deviations:
no
Remarks:
Not specified in report
GLP compliance:
not specified
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
no data
Oxygen conditions:
aerobic
Inoculum or test system:
mixture of sewage, soil and natural water
Details on inoculum:
Sludge was obtained from 10 locations (Fukogawa city sewage, Fukashiba industry sewage plant, Nakahama city sewage plant, Ochiai city sewage plant, Kitakami river, Shinano river, Yoshino river, Lake Biwa, Hiroshima bay and Dookai bay) four times each year. Inoculum is cultured in the laboratory on a glucose/peptone medium for 30-120 days prior to use in the test.
Duration of test (contact time):
14 d
Initial conc.:
100 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
Four test solutions were prepared: 1) water + test substance, 2) sludge + test substance, 3) sludge + aniline and 4) control blank.
Preliminary study:
no data
Test performance:
no data
Parameter:
% degradation (O2 consumption)
Value:
18
Sampling time:
14 d
Details on results:
no additional information available
Results with reference substance:
no additional information available

Epichlorohydrin was not readily biodegradable in this test.

Interpretation of results:
other: not readily biodegradable
Conclusions:
The biodegradability of epichlorohydrin was examined using the MITI test. Epichlorohydrin was not readily biodegradable in this test at a concentration (100 mg/l) where inhibition of the inoculum cannot be ruled out. No information on inhibitory effects of the test material on the inoculum is available.
Executive summary:

The biodegradability of epichlorohydrin was examined using the MITI test. Epichlorohydrin was not readily biodegradable in this test.

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: No data on GLP but was a guideline study.
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
Deviations:
not specified
GLP compliance:
not specified
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
no data
Oxygen conditions:
aerobic
Inoculum or test system:
mixture of sewage, soil and natural water
Details on inoculum:
Sludge was obtained from 10 locations (Fukogawa city sewage, Fukashiba industry sewage plant, Nakahama city sewage plant, Ochiai city sewage plant, Kitakami river, Shinano river, Yoshino river, Lake Biwa, Hiroshima bay and Dookai bay) four times each year. Inoculum is cultured in the laboratory on a glucose/peptone medium for 30-120 days prior to use in the test.
Duration of test (contact time):
14 d
Initial conc.:
100 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
Four test solutions were prepared: 1) water + test substance, 2) sludge + test substance, 3) sludge + aniline and 4) control blank.
Preliminary study:
no data
Parameter:
% degradation (O2 consumption)
Value:
68
Sampling time:
14 d
Parameter:
% degradation (TOC removal)
Value:
84.3
Sampling time:
14 d
Remarks on result:
other: Test terminated after 14 d.
Parameter:
% degradation (test mat. analysis)
Value:
92.5
Sampling time:
14 d
Remarks on result:
other: Test terminated after 14 d.
Details on results:
no additional information provided
Results with reference substance:
Result for references substance not given in the MITI database. For the study results to be accepted, the percentage biodegradation of aniline has to be >40 and >60% in 7 and 14 days, respectively.

The test material, 3 -chloro-1,2 -propylene glycol, meets the definition of readily biodegradable in the OECD 301C test.

Interpretation of results:
readily biodegradable
Conclusions:
The test material, 3 -chloro-1,2 -propylene glycol, meets the definition of readily biodegradable in the OECD 301C test.
Executive summary:

The test material, 3 -chloro-1,2 -propylene glycol, which is the initial degradation product of epichlorohydrin, meets the definition of readily biodegradable in the OECD 301C test.

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
not specified
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: The study was not conducted in accordance to any guidelines and is a non GLP study.
Principles of method if other than guideline:
Ready biodegradability study using non-acclimated organisms over an observation period of 20 days and comparison of BOD and ThOD as parameter.
GLP compliance:
not specified
Oxygen conditions:
aerobic
Remarks:
no additional information specified
Inoculum or test system:
activated sludge, non-adapted
Details on inoculum:
no data
Duration of test (contact time):
20 d
Parameter:
% degradation (O2 consumption)
Value:
48
Sampling time:
20 d
Remarks on result:
other: After 5, 10 and 15 days, 4, 33 and 36%, respectively, of the theoretical oxygen consumption was used.
Details on results:
Theoretical oxygen consumption was 1.21

Using unadapted mirco-organisms, 48% of the epichlorohydrin was biodegraded in 20 days.

Conclusions:
Using non-adapted organisms, 48% of the epichlorohydrin was biodegraded in 20 days based on BOD/ThOD.
Executive summary:

Using non-adapted organisms, 48% of the epichlorohydrin was biodegraded in 20 days based on BOD/ThOD.

Description of key information

The hydrolysis product of Epichlorohydrin is readily biodegradable. The Japanese Institute of Technology and Evaluation concludes Epichlorohydrin to be readily biodegradable based on this result.

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable
Type of water:
freshwater

Additional information

Biodegradability of Epichlorohydrin has been investigated following the OECD 301 C (MITI) protocol at 100 mg/l; a concentration that may be inhibitory to the inoculum (no information on inhibition available). However, the hydrolysis product of Epichlorohydrin is readily biodegradable. The Japanese Institute of Technology and Evaluation concludes Epichlorohydrin to be readily biodegradable based on this result.