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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Link to relevant study record(s)

Description of key information

All available data (reliable and not rated studies included):
Daphnia magna (mg/l): EC50(24hrs): 3090, 2500, 2306 (reported as range finder in long term toxicity testing). EC50(48hrs): 610, 799, 742. EC50 (unknown test time): 590.
Daphnia pulex (mg/l): EC50 (48hr): 260
Daphnia Cucullata:(mg/l): EC50 (48hr) 165.
Gammarus pulex (mg/l); EC50 (4hr) 750
Hydra Oligactis (mg/l): EC50 (48hr) 1350. NOEC (48hr): 1120
Artemia salina )mg/l): TLm (24hr): 1590, IC50 (24hr, 25% saltwater) 346, IC50 (24hr, 50% saltwater) 655
Aquatic mollusc (unspecified) (mg/l): LC50 (48hr) 1100
Aquatic worm (unspecified) (mg/l): EC50 (48hr) 1200, 3020

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
165 mg/L

Marine water invertebrates

Marine water invertebrates
Effect concentration:
346 mg/L

Additional information

A total of 15 results from 8 different species are available from all studies identified. Two reliable 24-hour studies with the pelagic invertebrate Daphnia magna Straus were conducted using the DIN 38412 method and reported 24-h EC50values of 2500 to 3090 mg/L. Lower LC50 values have been reported in Daphnia magna but, but in each case there were a number of limitations with these studies precluding their designation as reliable for use. Lower values were also reported in Daphnia pulex (230 -290 mg/L) and in Daphnia cucullata (154 -175 mg/L) and an LC50 value of 590 mg/L in Gammarus pulex was reported in the same studies, but again only limited information is available. 24-h EC50 values in the marine invertebrate Artemia salina of 1590 mg/L and 349 -645 have been reported.  EC50values reported for a number of other species were invariably above 1000 mg/l.

The key parameter would normally be based on the reliable studies available. However, since these represent some of the higher values available and appear to be from a less sensitive species, the results from the supporting studies cannot be ignored, even though these are from studies whose reliability cannot be confirmed. As a conservative approach therefore, the key parameter is based on the lowest value reported, which is an EC50 of 165mg/l reported in Daphnia cucullata. Similarly, for salt water species, the key parameter is based on the EC50 of 346mg/l reported in 25% strength seawater again from an unratable study.