Amides, C8-18 (even numbered) and C18-unsatd., N,N-bis(hydroxyethyl)

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

November 7 to November 17, 1994

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

No analytical dose verification.

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

yes

Remarks:

no analytical verification of test conc was done

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

The test solution was prepared in dilution water. The test material was found to be soluble up to 1000 mg/L in water.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Strain: Not specified
- Source: Toxicon's Daphnia culture
- Age at study initiation: Newly hatched, 6 to 24 h old
- Weight at study initiation: Not specified
- Length at study initiation: Not specified

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

none

Hardness:

Not specified

Test temperature:

21+/-1°C

pH:

7.8+/-0.2

Dissolved oxygen:

Not specified, initial oxygen saturation > 90%

Salinity:

250+/-25 mg CaCO3/L

Nominal and measured concentrations:

- Nominal: 0, 1, 2, 4, 8 and 16 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type: open
- Material, size, headspace, fill volume: Glass petri dishes with 50 mL solution
- Aeration: The dilution water was initially aerated to an oxygen saturation value of > 90%
- Renewal rate of test solution: Not applicable, static test
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): Two
- No. of vessels per control (replicates): Two


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reverse osmosis water

Composition of dilution water: According to ISO test water composition



OTHER TEST CONDITIONS
- Adjustment of pH: When required, it was done with HCl or NaOH
- Photoperiod: 16 h/8 h light: dark
- Light intensity: Subdued, no oher details


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Immobilisation in the control and test animals at 24 and 48 h


TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2

Reference substance (positive control):

not required

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

ca. 3.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

ca. 1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

ca. 2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

Control dish: Less than 10% immobility was observed in the control at 24 and 48 h.
Test dish: At 16 and 8 mg/L, 100% immobilisation was observed.
Oxygen saturation: > 97% oxygen saturation was maintained at the end 48 h. The pH was maintained around 8.0.
For details please refer to the attached results table in the 'background material' section.

None.

Validity criteria fulfilled:

yes

Remarks:

Both the criteria for dissolved oxygen and control immobility were fulfilled

Conclusions:

Under the study conditions, the nominal 48 h EC50 was determined to be 3.2 mg/L. The nominal 48 h LOEC and NOEC values were 2.0 and 1.0 mg/L, respectively.

Executive summary:

A study was conducted to determine the acute toxicity of the test substance, C8-18 and C18-unsatd. DEA, to Daphnia magna according to OECD Guideline 202, in compliance with GLP. The daphnids were exposed to test substance (directly diluted in test medium) at 0, 1, 2, 4, 8 and 16 mg/L for 48 h under static conditions. Observations were performed at 0, 24 and 48 h to determine the % of immobilised daphnids in the control and test vessels. No analytical dose verification was conducted. Under the study conditions, the nominal 48 h EC50 was determined to be 3.2 mg/L. The nominal 48 h LOEC and NOEC values were 2.0 and 1.0 mg/L, respectively (Olsson, 1994).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

3.2 mg/L

Additional information

A study was conducted to determine the acute toxicity of the test substance, C8-18 and C18-unsatd. DEA, to Daphnia magna according to OECD Guideline 202, in compliance with GLP. The daphnids were exposed to test substance (directly diluted in test medium) at 0, 1, 2, 4, 8 and 16 mg/L for 48 h under static conditions. Observations were performed at 0, 24 and 48 h to determine the % of immobilised daphnids in the control and test vessels. No analytical dose verification was conducted. Under the study conditions, the nominal 48 h EC50 was determined to be 3.2 mg/L. The nominal 48 h LOEC and NOEC values were 2.0 and 1.0 mg/L, respectively (Olsson, 1994).