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Ecotoxicological information

Short-term toxicity to fish

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Description of key information

The acute 96 hour LC50 values for freshwater fish range from 2.4 to 4.9 mg/L.

Key value for chemical safety assessment

LC50 for freshwater fish:
2.4 mg/L

Additional information

A study was conducted to evaluate the acute toxicity of amides, C8-18 and C18-unsatd., N,N-bis(hydroxyethyl) to rainbow trout(Oncorhynchus mykiss)under semi-static conditions. The procedures followed OECD guideline 203. Based on the results of a range-finding study, rainbow trout (20) were exposed to the test substance in a concentration range of 1.0, 1.8, 3.2, 5.6 and 10 mg/L for 96 hours. Mortality and adverse reactions were determined at 3, 6, 24, 48, 72 and 96 hours. Based on the results, the 96 hLC50 and NOEC were determined to be 2.4 and 1.0 mg/L, respectively (Wetton PM, 1996).

A study was carried out to determine the acute toxicity of amides, C8-18 and C18-unsatd., N,N-bis(hydroxyethyl) to zebra fish according to OECD guideline 203. Ten fish per groups were exposed to the test substance at 0, 1.0, 2.0, 4.0, 6.0 and 8.0 mg/L under semi-static conditions. Test medium was renewed every 24 hours. Mortality was observed at 3, 24, 48, 72 and 96 hours. 100% mortality was observed at the highest concentration by 24 hours and at 6.0 mg/L by 96 hours. There was a single mortality at 4.0 mg/L by 96 hours and no mortality at the two lower doses. The 96 h LC50and NOEC were 4.9 and 2.0 mg/L, respectively (Olsson T, 1994).