Aniline

Dermal absorption

Administrative data

Endpoint:

dermal absorption in vitro / ex vivo

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Data source

Reference

Reference Type:

publication

Title:

Enhancement of percutaneous penetration of aniline and o-toluidine in vitro using skin barrier creams

Author:

Korinth

Year:

2008

Bibliographic source:

Toxicology in vitro 22, 812-818

Materials and methods

Test material

Test material information

Constituent 1

Reference substance name:

Aniline

EC Number:

200-539-3

EC Name:

Aniline

Cas Number:

62-53-3

Molecular formula:

C6H7N

IUPAC Name:

aniline

Results and discussion

Absorption in different matrices:

Aniline (ANI) and the human carcinogen o-toluidine (OT) are released at the workplace during the production and processing of
rubber. Recently, we showed in rubber industry workers that a frequent use of skin barrier creams (SBC) increased the internal exposure
of ANI and OT. In the present study, diffusion cells were used to investigate the effects of two SBC and one skin care cream (SCC) on
percutaneous penetration of neat ANI and OT as well as of OT from a mixture with a workplace specific lubricant. The experiments were
carried out with untreated and with skin creams treated human skin. A considerable percutaneous penetration enhancement of test compounds
was observed for treated skin compared with untreated skin; the highest enhancement (mean factors 6.2–12.3) was found for SBC
(based on oil in water emulsion) treated skin. The lowest penetration enhancement showed SCC treated skin (mean factors 4.2–9.7). The
in vitro data support our findings in workers that the percutaneous absorption of aromatic amines significantly increases in presence of
skin creams. The efficacy of skin creams to protect the percutaneous penetration of aromatic amines is not confirmed by our own
experiments.

Applicant's summary and conclusion

Executive summary:

Aniline (ANI) and the human carcinogen o-toluidine (OT) are released at the workplace during the production and processing of rubber. Recently, we showed in rubber industry workers that a frequent use of skin barrier creams (SBC) increased the internal exposure of ANI and OT. In the present study, diffusion cells were used to investigate the effects of two SBC and one skin care cream (SCC) on percutaneous penetration of neat ANI and OT as well as of OT from a mixture with a workplace specific lubricant. The experiments were carried out with untreated and with skin creams treated human skin. A considerable percutaneous penetration enhancement of test compounds was observed for treated skin compared with untreated skin; the highest enhancement (mean factors 6.2–12.3) was found for SBC (based on oil in water emulsion) treated skin. The lowest penetration enhancement showed SCC treated skin (mean factors 4.2–9.7). The in vitro data support our findings in workers that the percutaneous absorption of aromatic amines significantly increases in presence of skin creams. The efficacy of skin creams to protect the percutaneous penetration of aromatic amines is not confirmed by our own experiments.