Butan-1-ol

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1979

Report date:

1979

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Principles of method if other than guideline:

Rats (10 males and 10 females per dose) were exposed for 4 hours to 6.58 or 17.76 mg/l of the test substance. A dynamic inhalation method with whole-body exposure and with analytical and nominal determination of the concentration was used. The animals were observed for 14 days.

GLP compliance:

no

Test type:

standard acute method

Test material

Test material information

Constituent 1

Reference substance name:

Butan-1-ol

EC Number:

200-751-6

EC Name:

Butan-1-ol

Cas Number:

71-36-3

Molecular formula:

C4H10O

IUPAC Name:

butan-1-ol

Test material form:

liquid

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: WIGA, Germany
- Weight at study initiation: 185 +- 15 g
- Housing: during exposure: 5 animals/cage
- Diet: ad libitum (food was withdrawn during the exposure)
- Water: ad libitum (water was withdrawn during the exposure)

ENVIRONMENTAL CONDITIONS
no data

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

other: air

Details on inhalation exposure:

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: Whole body inhalation system
- Exposure chamber volume: 200 L
- Method of holding animals in test chamber: animals are kept in wire mesh cages within the exposure chamber (5 animals/cage)


TEST ATMOSPHERE
- Brief description of analytical method used: samples were taken from the exposure chamber and analyzed via gaschromatography
- Samples taken from breathing zone: yes

Analytical verification of test atmosphere concentrations:

yes

Remarks:

via gaschromatography

Duration of exposure:

4 h

Concentrations:

6.58, 17.76 mg/L

No. of animals per sex per dose:

10

Control animals:

yes

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: the animal weight was determined before the start of the exposure, after 7 days and at the end of the observation period. The animals were checked daily for clinical signs and mortality
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight

Statistics:

no data

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

LC0

Effect level:

> 17.76 mg/L air (analytical)

Exp. duration:

4 h

Remarks on result:

other: highest concentration that could be generated

Mortality:

no deaths occured

Clinical signs:

other: none observed

Body weight:

Only marginal differences (slightly reduced weight gain) were observed between the treated groups and the control group. No dose dependency was observed.

Gross pathology:

no abnormalities detected