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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
toxicity to other aquatic vertebrates
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Meets generally accepted standards.

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1972

Materials and methods

Test guideline
Qualifier:
no guideline followed
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Methyl acetate
EC Number:
201-185-2
EC Name:
Methyl acetate
Cas Number:
79-20-9
Molecular formula:
C3H6O2
IUPAC Name:
methyl acetate

Sampling and analysis

Analytical monitoring:
not specified

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Rana pipiens
Details on test organisms:
TEST ORGANISM
- Common name: Northern Leopard Frog
- Source: Commercial supplier
- Age at study initiation: Tadpoles
- Length at study initiation: Between 2.5 and 3.5 inches, corresponding to 6.4 and 8.9 cm

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Remarks on exposure duration:
not reported

Test conditions

Test temperature:
20°C
Details on test conditions:
TEST SYSTEM
- Test vessel: Glass aquaria filled with 500 ml tap water containing various concentrations of the test substance
- No. of organisms per vessel: 10

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap water. No details on further preparation reported

EFFECT PARAMETERS MEASURED : Narcosis
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Dose descriptor:
other: Threshold narcotic concentration defined as that concentration at which touching the tadpoles failed to cause motion
Effect conc.:
157 mmol/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: test result
Dose descriptor:
other: Threshold narcotic concentration defined as that concentration at which touching the tadpoles failed to cause motion
Effect conc.:
11 630.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: test result
Dose descriptor:
other: Threshold narcotic concentration defined as that concentration at which touching the tadpoles failed to cause motion
Effect conc.:
>= 70 - <= 90 mmol/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: Data from Overton (1901) as cited in the publication converted to mg/L
Dose descriptor:
other: Threshold narcotic concentration defined as that concentration at which touching the tadpoles failed to cause motion
Effect conc.:
>= 5 185.6 - <= 6 667.2 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: Data from Overton (1901) as cited in the publication, converted to mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Executive summary:

The narcotic potency of methyl acetate to tadpoles of Rana pipiens was assessed in a static system. Tadpoles were exposed to various concentrations of the substance and the threshold narcotic concentration was determined as the respective concentration at which tadpoles failed to cause motion. For methyl acetate this value was determined to be 157 mmol/l corresponsing to 11630.6 mg/l.