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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
22-Jan-2004 to 24-Jan-2004
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP compliant, guideline, available as an unpublished report, fully acceptable, no restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2003
Report Date:
2004

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
yes
Remarks:
The concentration of the test substance in solution was not determined prior to use. Due to the limited solubility of the test substance, it may not have been possible to demonstrate that the initial concentration was maintained throughout the test. It wa
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Details on test material:
EMBSI Identification: MRD-03-769 Pyrolysis C10+ Fuel Oil. CAS number 68513-69-9 and 68921-67-5
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):no data

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Water quality measurements performed on Day 0 and at termination. Observations for immobilization of daphnids were made at 24 and 48 hours. No undissolved test substnace was observed in any of the test chambers.

Test solutions

Vehicle:
no
Details on test solutions:
Individual treatments were prepared by adding test substance to 12.0L of vehicle/dilution water in glass aspirator bottles and stirring for 24hrs. WAFs were removed after one hour without stirring. All treatments appeared clear and colorless.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
cultured in house. <24 hours old from 15 day old parentsnot fed during the study

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
none

Test conditions

Hardness:
144 mg/L as CaCO3
Test temperature:
20.1°C (S.D. = 0.1)
pH:
8.1 to 8.3
Dissolved oxygen:
7.9 to 8.1 mg/L
Salinity:
NA
Nominal and measured concentrations:
Measured concentrations: 0, 0.07, 0.14, 0.82, 1.7, 4.2Actual loading rates: Control, 0.17, 0.33, 0.83, 1.8, 4.1
Details on test conditions:
Individual Water Accommodated Fractions were prepared for each treatment.125ml Erlenmeyer flasksNo headspace with no aeration5 daphnids in each replicates4 replicates per concentrationDiurnal light: approximately 16 hours light and 8 hours dark.91 to 135 lux during full daylight periods.

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
1.2 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 99% CL 0.82-1.7
Duration:
48 h
Dose descriptor:
EL50
Effect conc.:
1.2
Nominal / measured:
nominal
Conc. based on:
other: loading rate
Basis for effect:
mobility
Remarks on result:
other: 99% CL 0.83-1.8
Details on results:
EL50-24 hr: 2.7, EC50-24 hr: 2.7
Results with reference substance (positive control):
no data
Reported statistics and error estimates:
The 24- and 48-hour EL50 and EC50 values were determined using a binomial method.

Any other information on results incl. tables

no further details

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
After Daphnia magna were exposed to WAFs prepared from Pyrolysis C10+ Fuel Oil for 48 hours, the EL50 was 1.2 mg/L and EC50 was 1.2 mg/L.
Executive summary:

After Daphnia magna were exposed to WAFs prepared from Pyrolysis C10+ Fuel Oil for 48 hours, the EL50 was 1.2 mg/L and EC50 was 1.2 mg/L. This study was GLP compliant and followed a standard guideline. It is considered reliable and suitable for use as a key study.