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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
12-Jan-2004 to 16-Jan-2004
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: GLP-compliant, guideline study, available as an unpublished report, acceptable with restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2003
Report Date:
2004

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Details on test material:
Pyrolysis C10+ Fuel Oil, CASRN 68513-69-9 and 68921-67-5Liquid under ambient laboratory conditions and immiscible in water and methanol but miscible in hexane. Initial density was measured (at 20°C) as 0.972 g/ml and final density was 0.973 g/ml.
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):no data

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Samples taken (with no headspace) Day 0, Day 1, Day 3 and Day 4, prior to renewals and in a composite of the replicates after each renewal. Observations were performed at 3, 6, 24, 48, 72 and 96 hrs after the beginning of the test

Test solutions

Vehicle:
no
Details on test solutions:
Treatments prepared by adding the appropriate amount of substance to 18.0L of dilution water in glass aspirator bottles. The mixtures were stirred for 24hrs. All treatments appeared clear/colourless with the test substnace floating at the surface. Mixtures were cooled for one hour without stirring before WAFs were removed.

Test organisms

Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
Thomas Fish Company, Anderson, CA, USA. Lot 482. Receipt date: 30-Dec-03For the 13 days prior to use the fish were observed for disease and parasites (for which they had not previously been treated). They were kept at approximately 14.1 °C in dilution water which was aerated continuously to provide a DO concentration of at least 80%. They were held under filtrated static conditions and were fed with Finfish Starter at least 5 days per week. There were no observed mortalities. 72 fish, 29 days post hatch. Mean weight: 0.206 g. Mean total length: 3.1 cm.

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Post exposure observation period:
none

Test conditions

Hardness:
104 mg/L as CaCO₃
Test temperature:
13.6 °C (mean) (S.D.=0.1)
pH:
7.3 to 8.1
Dissolved oxygen:
6.8 to 8.6 mg/L
Salinity:
NA
Nominal and measured concentrations:
0, 0.40, 0.79, 1.7, 3.7 and 6.3 (mg/L, measured) plus the controlActual loading rate (mg/l): 0, 0.47, 0.90, 1.8, 3.5 and 7.0
Details on test conditions:
Reconstituted UV-sterilized, deionized, well water (Batch 290)4L aspirator bottles containing approx 4.5L test solution (no headspace)AerationDaily renewal4 organisms per vessel3 replicates per concentrationBiomass loading: 0.183 g of fish/L16 hours light; 8 hours dark607 to 614 Lux during full daylight periods.Fish were not fed during the study, nor for 24 hours prior to commencement.

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LL50
Effect conc.:
1.1 mg/L
Nominal / measured:
nominal
Conc. based on:
other: actual loading rate
Basis for effect:
mortality
Remarks on result:
other: 95% CL 1.0-1.3
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
1 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 95% 0.9-1.2
Details on results:
Results have been expressed as the lethal loading/lethal concentration which result in 50% mortality compared to the control for the specified time of exposure
Results with reference substance (positive control):
na
Reported statistics and error estimates:
LL50 and LC50 (24-96 hour) were determined using a Trimmed Spearman-Karber Method.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The 96-hour LC50 was reported to be 1.0 mg/L.
Executive summary:

The 96-hour LL50 was reported to be 1.1 mg/L. whilst the LC50 was 1mg/L. This study is GLP compliant and follows a standard guideline. It is considered reliable and suitable for use as a key study.