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Biodegradation in water: screening tests

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Description of key information

Readily biodegradable: 81% in 28 days (OECD TG 301F), 1.160.000 mg/L (COD) German DIN 38409 T.41

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information

The ready biodegradation of the substance was investigated in a study conducted according to OECD Guideline 301F (Ready Biodegradability: Manometric Respirometry Test) and EU Method C.4-D (Determination of the “Ready” Biodegradability – Manometric Respirometry Test) over a period of 28 days and using domestic activated sludge as inoculum. The biodegradation rate was determined by measurements of oxygen consumption. Inoculum blank, abiotic control, procedural/functional control with the reference substance sodium benzoate, and toxicity control using 100 mg/L test item and 100 mg/L reference compound were performed.

This study is regarded as reliable without restriction and satisfies the guideline requirements for ready biodegradation.

The test item proved to be readily biodegradable and fulfilling the 10-d window criterion. The functional control reached the pass level >60 % after 14 d. In the toxicity control containing both test and reference item 62 % biodegradation based on oxygen consumption occurred within 14 d thus indication that the test item was not inhibitory to the inoculum at the concentrations tested.

The COD of the substance was determined according to the German DIN 38409 T.41. A COD of 1.160.000 mg/L was determined (calculated on the basis of the available product).

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