Registration Dossier

Administrative data

Endpoint:
acute toxicity: other routes
Type of information:
experimental study
Adequacy of study:
other information
Study period:
08. Mar 1966 - 21. Mar 1966
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable, well-documented study report which meets basic scientific principles.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1966
Report date:
1966

Materials and methods

Principles of method if other than guideline:
other: BASF-Test
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
1,1',1''-nitrilotripropan-2-ol
EC Number:
204-528-4
EC Name:
1,1',1''-nitrilotripropan-2-ol
Cas Number:
122-20-3
Molecular formula:
C9H21NO3
IUPAC Name:
1-[bis(2-hydroxypropyl)amino]propan-2-ol
Test material form:
solid
Specific details on test material used for the study:
- Analytical purity: ca. 92-95 %
- Impurities (identity and concentrations): ca. 3-6 % Diisopropanolamine; < 1 % Monoisopropanolamine

Test animals

Species:
mouse
Strain:
other: Kisslegg-mice
Sex:
male/female

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
water
Doses:
200, 400, 800, 1000, 1250, 1600 mg/kg
No. of animals per sex per dose:
5
Control animals:
no

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
ca. 1 400 mg/kg bw
Mortality:
1600 mg/kg: all animals died within the first 24 h.
1250 mg/kg: 1 female died within 24 h.
Clinical signs:
1600 mg/kg:
15 min post application: abdominal position, fluffed fur, intermittent respiration.
25 min post application: slight tremulous convulsions, abdominal position, accelerated respiration, mouth discharge, partly dyspnoea.

1250 and 1000 mg/kg:
15 min post application: staggering, accelerated respiration, abdominal position, flattened ears.
1 and 2 days post application: heavy ruffled fur, accelerated respiration, high stepping tremulous gait.
3 and 4 days post application: accelerated respiration, high stepping tremulous gait.

800 and 400 mg/kg:
10 min post application: high stepping, hoppling gait, accelerated respiration, gaunted sides, flattened ears, animals don´t continue in abominal position.
5 h and 1 day post application: abdominal position, heavy fluffed fur, gaunted sides, very calm attitude.

200 mg/kg: 5 h post application fluffed fur and accelerated respiration.
Gross pathology:
1250 mg/kg: 6x agglutination of intestinal loops.
800 mg/kg: 2x agglutination of intestinal loops.

Any other information on results incl. tables

Mortality:

Dose (mg/kg)  Conc. (%)  1 h  24 h  48 h  7 days  14 days
 1600  20  0/10  10/10  10/10  10/10  -
 1250  8  0/10  1/10  1/10  1/10  -
 1000  8  0/10  0/10  0/10  0/10  -
 800  8  0/10  0/10  0/10  0/10  -
 400  8  0/10  0/10  0/10  0/10  -
 200  2  0/10  0/10  0/10  0/10  -

Applicant's summary and conclusion