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EC number: 201-545-9 | CAS number: 84-61-7 DCHP
The potential of the test item, DICYCLOHEXYLPHTHALATE, to cause corrosion/severe irritation by using the Bovine Corneal Opacity and Permeability (BCOP) assay, was examined in agreement with OECD Guideline no. 437 (adopted on) and OECD Supplement to Test Guidelines nos. 437 and 438.
The test item was tested suspended at 20% (w/v) in physiological saline (being a solid non surfactant) on the epithelial surface of three idoneous bovine corneas, for an exposure period of 4 hours.
Positive and negative controls [a 20% (w/v) Imidazole solution in physiological saline and physiological saline alone, respectively] were concurrently tested in similar condition.
The mean opacity detected with an opacitometer at the end of the test item exposure period was 0.1, comparable to the negative control.
After the determination of opacity, the epithelial surface was treated with a 0.5% solution of sodium fluorescein in DPBS for 90 minutes, to investigate alteration in cornea permeability.Mean OD490value of the corneas treated with the test item was unaffected by treatment, as well as the negative control.Negative and positive controls gave the expected results.
The results obtained indicate that the test item did not induce changes in both cornea opacity and permeability under the reported experimental conditions. The calculatedin vitroirritancy score (IVIS) for the test item is 0.
According to the OECD Guideline no. 437, the test item is not to be classified as corrosive or severely irritant to the eye.
Moreover, according to the criteria stated in theOECD Supplement to Test Guidelines nos. 437 and 438, there is no indication of irritant effect to the eye.
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